NDLI: Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato

Content Provider	Springer Nature Link
Author	González Cabezuelo, José M. Capel, Juan Abad, Jesús Tomás, Diego M. Fernández Muñoz, Rafael Moriones, Enrique Loza, Rafael
Copyright Year	2012
Abstract	The tomato yellow leaf curl virus (TYLCV), transmitted by whitefly, causes major disease losses to tomato crops in tropical and subtropical regions of the world. Several genes conferring resistance to TYLCV, mainly Ty-1 and Ty-3 genes, have been introgressed to cultivated tomato (Solanum lycopersicum) from the wild relative species Solanum chilense. By combining bulked segregant analysis and amplified fragment length polymorphisms (AFLP), several AFLP markers closely linked to Ty-1 and Ty-3 genes were identified from the resistant breeding line TZ841-4. Cloning and sequencing of the selected AFLP fragments allowed us to develop codominant cleaved amplified polymorphic sequence and dominant sequence characterized amplified region markers closely linked to Ty-1. In addition, Ty-3-linked allelic-specific markers have been discriminated by a quantitative real-time PCR protocol. Taken together, these markers constitute useful tools for marker-assisted selection breeding programs to improve genetic resistance to TYLCV, and also to initiate map-based cloning approaches to isolate the resistance genes.
Starting Page	1131
Ending Page	1142
Page Count	12
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	30
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-02-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Disease resistance TYLCV Marker-assisted selection AFLP SCAR CAPS Plant Physiology Biotechnology Plant Genetics & Genomics Plant Pathology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm

Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato

Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA

Tomato yellow leaf virus (TYLCV): The structure, ecotypes and the resistance germplasm resources in tomato

Use of Tomato yellow leaf curl virus (TYLCV) Rep Gene Sequences to Engineer TYLCV Resistance in Tomato.

Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato

Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato

Similar Documents

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm

Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato

Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA

Tomato yellow leaf virus (TYLCV): The structure, ecotypes and the resistance germplasm resources in tomato

Use of Tomato yellow leaf curl virus (TYLCV) Rep Gene Sequences to Engineer TYLCV Resistance in Tomato.

Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato

Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato