NDLI: Transformation of Nicotiana tabacum with a native cry1Ia5 gene confers complete protection against Heliothis armigera

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Molecular Breeding : Volume 4, Issue 5, October 1998

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Molecular Breeding : Volume 3

Transformation of Nicotiana tabacum with a native cry1Ia5 gene confers complete protection against Heliothis armigera

Content Provider	Springer Nature Link
Author	Selvapandiyan, Angamuthu Reddy, Vanga S. Kumar, P. Anand Tewari, Krishna K. Bhatnagar, Raj K.
Copyright Year	1998
Abstract	A cry1Ia5 insecticidal toxin coding gene has been cloned from an Indian isolate of Bacillus thuringiensis. Sequence analyses of the cry1Ia5 gene revealed the absence of potential polyadenylation signal sequences thus making it a suitable candidate for expression in plants without extensive modification. This possibility was examined by subcloning the cry1Ia5 gene into a plant expression vector and then transferring it to Nicotiana tabacum through Agrobacterium-mediated transformation. Our results demonstrate that N. tabacum with a stably integrated native cry1Ia5 gene afforded complete protection against predation by Heliothis armigera. Forty three percent of the transgenic plants displayed a high level of protection against insect predation. The protection obtained in transgenic plants with the cry1Ia5 gene was comparable to that obtained with the synthetically modified cry1A(b) or cry1A(c) genes. The results demonstrate that novel insecticidal genes already exist in nature that do not require extensive modifications for efficient expression in plants.
Starting Page	473
Ending Page	478
Page Count	6
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	4
Issue Number	6
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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Transformation of Nicotiana tabacum with a native cry1Ia5 gene confers complete protection against Heliothis armigera

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Transformation of Nicotiana tabacum with a native cry1Ia5 gene confers complete protection against Heliothis armigera