NDLI: Construction and characterization of a BIBAC library of Jatropha curcas L. and identification of BIBAC clones containing genes associated with fatty acid metabolism

Content Provider	Springer Nature Link
Author	Fu, Yuhua Scheuring, Chantel F. Wang, Haiyan Zhang, Hong bin Wang, Wenquan
Copyright Year	2010
Abstract	Jatropha curcas L. is a potentially significant bioenergy crop in the tropics and subtropics. Here we present a plant-transformation-competent binary bacterial artificial chromosome (BIBAC) library from Jatropha cultivar YN049-4. This library was constructed with BamH in the vector pCLD04541, consists of 30,720 clones and is arrayed in 80 384-well microtiter plates. Since 92.1% (28,293) of its clones were shown to contain Jatropha DNA inserts with an average size of 131.9 kb, the library is estimated to represent approximately 8.9 haploid genome equivalents of the species, thus providing a greater than 99% probability of discovering a particular single-copy sequence in the library. High-density clone filters were made from a subset of the library and hybridized with nine pairs of overgos designed from genes involved in fatty acid metabolism. Hybridization results showed that eight overgo pairs were able to identify positive clones from the subset of the library, with an average of 5.3 clones per probe, suggesting that it is suitable for Jatropha genomics and genetics research. Because this library, to our knowledge, represents the first large-insert, plant-transformation-competent BIBAC library for Jatropha, it will provide a vital resource for advanced genomics research, including isolation and characterization of genes and quantitative trait loci, integrative physical mapping and genome sequencing.
Starting Page	559
Ending Page	567
Page Count	9
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	28
Issue Number	4
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-09-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	BIBAC library Jatropha curcas L. Fatty acid metabolism Plant Physiology Plant Genetics & Genomics Plant Sciences Biotechnology Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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