NDLI: The patterns of utilization and accumulation of polyphosphates in the cytosol of the yeast Saccharomyces cerevisiae under inactivation of exopolyphosphatase genes PPX1 and PPN1

Content Provider	Springer Nature Link
Author	Lichko, L. P. Kulakovskaya, T. V. Kulaev, I. S.
Copyright Year	2009
Abstract	The effect of inactivation of the PPX1 and PPN1 genes encoding the yeast exopolyphosphatases on the activities of these enzymes and polyphosphate content in the cytosol of Saccharomyces cerevisiae was studied under P$_{i}$ deficit and P$_{i}$ excess in the cultivation medium. Under P$_{i}$ deficit, exopolyphosphatase activity in strain CRN (with inactivated PPN1 gene) and in the parent strain CRY increased 3- and 1.5-fold, respectively. In the strain CRX (with inactivated PPX1 gene), exopolyphosphatase activity did not change under P$_{i}$ deficit. Transfer from P$_{i}$-deficient to P$_{i}$-rich medium was accompanied by an ∼1.7-fold increase of exopolyphosphatase activities in the cytosol preparations of strains CRY, CRX, and CRN. In the cytosol of the double mutant, exopolyphosphatase activity was practically absent under all of the above cultivation conditions. The content of polyphosphates in the cytosol preparations of all strains under study substantially decreased under P$_{i}$ deficit. Transfer from P$_{i}$-deficient to P$_{i}$-rich medium was accompanied by polyphosphate over-accumulation only in the cytosol preparations of stains CRX and CNX, where their levels increased ∼1.3 and 3.5-fold, respectively. No over-accumulation was observed in the parent strain CRY and in the PPN1-deficient strain CRN. These data suggest that the exopolyphosphatases encoded by the PPX1 and PPN1 genes are not involved in polyphosphate synthesis.
Starting Page	304
Ending Page	307
Page Count	4
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	78
Issue Number	3
e-ISSN	16083237
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2009-06-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	polyphosphates exopolyphosphatases cytosol PPX1 and PPN1 mutants Saccharomyces cerevisiae Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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Inactivation of PPX1 and PPN1 genes encoding exopolyphosphatases of Saccharomyces cerevisiae does not prevent utilization of polyphosphates as phosphate reserve

The effect of inactivation of the exo-and endopolyphosphatase genes PPX1 and PPN1 on the level of different polyphosphates in the yeast Saccharomyces cerevisiae

Inactivation of the PPN1 gene exerts different effects on the metabolism of inorganic polyphosphates in the cytosol and the vacuoles of the yeast Saccharomyces cerevisiae

Inorganic polyphosphates and exopolyphosphatases in different cell compartments of Saccharomyces cerevisiae

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Finding of endopolyphosphatase activity in the yeast Saccharomyces cerevisiae

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