NDLI: The Construction and Monitoring of Genetically Tagged, Plasmid-Containing, Naphthalene-Degrading Strains in Soil

Content Provider	Springer Nature Link
Author	Filov, A. E. Akhmetov, L. I. Puntus, I. F. Esikova, T. Z. Gafarov, A. B. Izmalkova, T. Yu. Sokolov, S. L. Kosheleva, I. A. Boronin, A. M.
Copyright Year	2005
Abstract	A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442(pNF142:: TnMod-OTc), has been constructed. The presence of the gfp gene (which codes for green fluorescent protein) and the kanamycin and rifampicin resistance genes in the chromosome of this strain allows the strain’s fate in model soil systems to be monitored, whereas a minitransposon, inserted into naphthalene biodegradation plasmid pNF142 and containing the tetracycline resistance gene, makes it possible to follow the horizontal transfer of this plasmid between various bacteria. Plasmid pNF142::TnMod-OTc is stable in strain P. putida KT2442 under nonselective conditions. The maximal specific growth rate of this strain on naphthalene is found to be higher than that of the natural host of plasmid pNF142. When introduced into a model soil system, the genetically tagged strain is stable and competitive for 40 days. The transfer of labeled plasmid pNF142::TnMod-OTc to natural soil bacteria, predominantly fluorescent pseudomonads, has been detected.
Starting Page	453
Ending Page	458
Page Count	6
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	74
Issue Number	4
e-ISSN	16083237
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2005-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	naphthalene biodegradation labeled plasmids conjugal transfer Pseudomonas Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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