NDLI: Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus

Please wait, while we are loading the content...

Components of antioxidant systems in the cells of aerotolerant sulfate-reducing bacteria of the genus Desulfovibrio (strains A2 and TomC) isolated from metal mining waste

Vanadate reduction under alkaline conditions by haloalkaliphilic Halomonas strains

Structural properties of capsular and O-specific polysaccharides of Azospirillum brasilense Sp245 under varying cultivation conditions

Phylogenetic diversity of microbial communities of the Posolsk Bank bottom sediments, Lake Baikal

Dynamics of the composition of a microbial consortium during start-up of a single-stage constant flow laboratory nitritation/anammox setup

Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus

Structural and functional organization of the plasmid regulons of Rhizobium leguminosarum symbiotic genes

Molecular phylogeny of pectinase genes PGU in the yeast genus Saccharomyces

Quantitative determination of indole-3-acetic acid in yeasts using high performance liquid chromatography—tandem mass spectrometry

Alkalitolerant micromycetes in acidic and neutral soils of the temperate zone

Effect of metal concentration on the microbial community in acid mine drainage of a polysulfide ore deposit

Antibiotic activity of bacterial endobionts of basidiomycete fruit bodies

Virus abundance in alluvial-sod soil determined by direct epifluorescence microscopy

Microbiology : Volume 85, Issue 5, September 2016

Microbiology : Volume 85, Issue 4, July 2016

Microbiology : Volume 85, Issue 3, May 2016

Microbiology : Volume 85, Issue 2, March 2016

Microbiology : Volume 85, Issue 1, January 2016

Microbiology : Volume 84

Microbiology : Volume 83

Microbiology : Volume 82

Microbiology : Volume 81

Microbiology : Volume 80

Microbiology : Volume 79

Microbiology : Volume 78

Microbiology : Volume 77

Microbiology : Volume 76

Microbiology : Volume 75

Microbiology : Volume 74

Microbiology : Volume 73

Microbiology : Volume 72

Microbiology : Volume 71

Microbiology : Volume 70

Microbiology : Volume 69

Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus

Content Provider	Springer Nature Link
Author	Tourova, T. P. Sokolova, D. Sh. Semeva, E. M. Shumkova, E. S. Korshuva, A. V. Babich, T. L. Poltaraus, A. B. Nazina, T. N.
Copyright Year	2016
Abstract	Ability to degrade crude oil n-alkanes was revealed in new strains of thermophilic bacilli isolated from petroleum reservoirs and a hot spring: Geobacillus toebii В-1024, Geobacillus sp. 1017, and Aeribacillus pallidus 8m3. The strains utilized С$_{10}$–С$_{30}$ n-alkanes (В-1024), С$_{10}$, C$_{11}$, and С$_{13}$–С$_{19,22}$ n-alkanes (1017), and C$_{11}$–C$_{29}$ n-alkanes (8m3). In all three strains, PCR amplification with specific degenerate oligonucleotide primers revealed the alkB gene encoding rubredoxin-dependent alkane monooxygenase. In strains В-1024 and 1017, fragments of the genes homologous to the ladA gene determining flavin-dependent alkane monooxygenase were also amplified. Nucleotide sequences of these genes were practically identical to those of the genes ladAα$_{B23}$, ladAβ$_{B23}$, and ladB $_{B23}$, which were revealed previously in Geobacillus thermoleovorans strain B23. For the latter strain, activity of respective enzymes in the oxidation of long-chain n-alkanes has been shown. Thus, simultaneous presence of the alkB and ladA genes coding alkane monooxygenases responsible for oxidation of medium-chain and long-chain n-alkanes in thermophilic bacilli was revealed for the first time.
Starting Page	693
Ending Page	707
Page Count	15
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	85
Issue Number	6
e-ISSN	16083237
Language	English
Publisher	Pleiades Publishing
Publisher Date	2016-12-02
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	alkB and ladA genes alkane monooxygenases Geobacillus Aeribacillus thermophilic bacteria oil degradation Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Detection and transcription of n-alkane biodegradation genes (alkB) in the genome of a hydrocarbon-oxidizing bacterium Geobacillus subterraneus K

Cloning and expression of three ladA-type alkane monooxygenase genes from an extremely thermophilic alkane-degrading bacterium Geobacillus thermoleovorans B23

Characterization of the aerobic hydrocarbon-oxidizing enrichments from a high-temperature petroleum reservoir by comparative analysis of DNA- and RNA-derived clone libraries

Identification of long-chain alkane-degrading (LadA) monooxygenases in Aspergillus flavus via in silico analysis

Hydrocarbon-oxidizing potential and the genes for n-alkane biodegradation in a new acidophilic mycobacterial association from sulfur blocks

Physiological and phylogenetic diversity of thermophilic spore-forming hydrocarbon-oxidizing bacteria from oil fields

Phylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkey

Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases

alkB homologs in thermophilic bacteria of the genus Geobacillus

Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus

Similar Documents

Detection and transcription of n-alkane biodegradation genes (alkB) in the genome of a hydrocarbon-oxidizing bacterium Geobacillus subterraneus K

Cloning and expression of three ladA-type alkane monooxygenase genes from an extremely thermophilic alkane-degrading bacterium Geobacillus thermoleovorans B23

Characterization of the aerobic hydrocarbon-oxidizing enrichments from a high-temperature petroleum reservoir by comparative analysis of DNA- and RNA-derived clone libraries

Identification of long-chain alkane-degrading (LadA) monooxygenases in Aspergillus flavus via in silico analysis

Hydrocarbon-oxidizing potential and the genes for n-alkane biodegradation in a new acidophilic mycobacterial association from sulfur blocks

Physiological and phylogenetic diversity of thermophilic spore-forming hydrocarbon-oxidizing bacteria from oil fields

Phylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkey

Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases

alkB homologs in thermophilic bacteria of the genus Geobacillus

Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus