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Degradation of natural rubber latex by new Streptomyces labedae strain ASU-03 isolated from Egyptian soil

Content Provider	Springer Nature Link
Author	Hesham, Abd El Latif Mohamed, Nadia H. Ismail, Mady A. Shoreit, Ahmed A. M.
Copyright Year	2015
Abstract	Natural rubber latex is one of the problems that raises the environmental concerns. In this study the degrading ability of Ficus elastica rubber latex by a bacterium strain ASU-03, isolated from Egyptian soil was assessed. The strain was able to produce clear zone around its colony on latex rubber containing medium and was identified by conventional methods as Streptomyces sp. Phylogenetic analysis of 16S rRNA (16S rRNA) and RNA polymerase ß-subunit (rpoB) genes were applied. Results of the 16S rRNA gene analysis revealed that the strain was highly related to Streptomyces sp. (100% similarity), so the rpoB gene was partially sequenced to clarify the specific name of the isolate. Phylogenetic tree based on rpoB gene sequences indicated that strain ASU-03 was highly similar to the reference strain Streptomyces labedae and both were shared a one cluster. The current results demonstrated that the use of a rpoB gene-based method gives a better resolution in the species level identification. To our knowledge, this species has never been reported to be involved in natural rubber degradation. This was therefore the first report about the degradation of Ficus elastic by S. labedae. The degradation of Ficus elastica rubber latex was determined by measuring the increase in protein content of bacterium (mg/g dry wt), reduction in molecular weight (g/mol) and inherent viscosity (dL/g) of the latex. Moreover the degradation was also confirmed by formation of aldehyde or keto group by Schiff’s reagent and by observing the growth of the Streptomyces strain using scanning electron microscopy.
Starting Page	351
Ending Page	358
Page Count	8
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	84
Issue Number	3
e-ISSN	16083237
Language	English
Publisher	Pleiades Publishing
Publisher Date	2015-06-09
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	streptomyces natural rubber latex biodegradation rpoB gene sequence 16S rRNA gene phylogenetic analysis Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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