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Influence of introducing the genetically modified strain Sinorhizobium meliloti ACH-5 on the structure of the soil microbial community

Content Provider	Springer Nature Link
Author	Androv, E. E. Petrova, S. N. Chizhevskaya, E. P. Korostik, E. V. Akhtemova, G. A. Pinaev, A. G.
Copyright Year	2009
Abstract	To study the influence of genetically modified microorganisms (GMM) on the number and structure of the soil microbial community, we introduced the genetically modified strain of Sinorhizobium meliloti into soil under controlled laboratory conditions. The analysis of the dynamics of soil microorganisms of all the main groups (archaea, bacteria, fungi) using the PCR with real-time detection and the analysis of the species structure of all the indicated components using T-RFLP were carried out for a month. The results of the quantitative PCR demonstrated that none of the components of the soil microbial community was appreciably influenced by the GMM introduced. The number of GMM decreased over a month more than 300-fold. The analysis of the dynamics of the eubacterial, archaeal, and fungal communities using T-RFLP did not detect fundamental changes in their structure.
Starting Page	474
Ending Page	482
Page Count	9
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	78
Issue Number	4
e-ISSN	16083237
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2009-08-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	genetically modified microorganism soil microbial communities T-RFLP Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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Influence of introducing the genetically modified strain Sinorhizobium meliloti ACH-5 on the structure of the soil microbial community

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