NDLI: Microbiological and Sybr® Green real-time PCR detection of major Fusarium head blight pathogens on wheat ears

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Microbiological and Sybr® Green real-time PCR detection of major Fusarium head blight pathogens on wheat ears

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Microbiological and Sybr® Green real-time PCR detection of major Fusarium head blight pathogens on wheat ears

Content Provider	Springer Nature Link
Author	Moradi, M. Oerke, E. C. Steiner, U. Tesfaye, D. Schellander, K. Dehne, H. W.
Copyright Year	2010
Abstract	Fusarium head blight (FHB) caused by several Fusarium species is one of the most serious diseases affecting wheat throughout the world. The efficiency of microbiological assays and real-time PCR to quantify major FHB pathogens in wheat ears after inoculation with F. graminearum, F. culmorum, F. avenaceum and F. poae under greenhouse and field conditions were evaluated. The frequency of infected kernel, content of fungal biomass, disease severity and kernel weight were determined. To measure the fungal biomass an improved DNA extraction method and a Sybr® Green real-time PCR were developed. The Sybr® Green real-time PCR proved to be highly specific for individual detection of the species in a matrix including fungal and plant DNA. The effect of Fusarium infection on visible FHB severity, frequency of infected kernels and thousand-kernel mass (TKM) significantly depended on the Fusarium species/isolate. F. graminearum resulted in highest disease level, frequency of infected kernels, content of fungal biomass, and TKM reduction followed by F. culmorum, F. avenaceum and F. poae, respectively. The comparison of frequency and intensity of kernel colonization proved differences in aggressiveness and development of the fungi in the kernels. Only for F. graminearum, the most aggressive isolate, application of microbiological and real-time PCR assays gave similar results. For the other species, the intensity of kernel colonization was lower than expected from the frequency of infection.
Starting Page	646
Ending Page	654
Page Count	9
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	79
Issue Number	5
e-ISSN	16083237
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-10-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	real-time PCR Fusarium head blight quantification Fusarium graminearum aggressiveness Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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