NDLI: Use of glucose and carbon isotope fractionation by microbial cells immobilized on solid-phase surface

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Use of glucose and carbon isotope fractionation by microbial cells immobilized on solid-phase surface

Content Provider	Springer Nature Link
Author	Zyakun, A. M. Kochetkov, V. V. Baskuv, B. P. Zakharchenko, V. N. Peshenko, V. P. Laurinavichius, K. S. Akhina, T. O. Siuva, T. V. Sizova, O. I. Boronin, A. M.
Copyright Year	2013
Abstract	By the example of glucose uptake by the soil bacteria Pseudomonas aureofaciens BS1393(pBS216) and Rhodococcus sp. 3–30 immobilized on a solid-phase surface (quartz sand), their growth parameters were determined: growth rate (doubling time), total CO$_{2}$ production, CO$_{2}$ production per cell, lag period with respect to substrate uptake, respiratory quotient. The growth of P. aureofaciens and Rhodococcus sp. on glucose revealed (1) differences of the lag period with respect to substrate (lag time of ∼4 h for P. aureofaciens and ∼26 h for Rhodococcus sp.); (2) differences between the maximal rates of CO$_{2}$ production (∼50 μg C-CO$_{2}$ g$^{−1}$ sand h$^{−1}$ for P. aureofaciens and ∼8.5 μg C-CO$_{2}$ g$^{−1}$ sand h$^{−1}$ for Rhodococcus sp.); (3) differences in CO$_{2}$ production per cell (∼1.94 × 10$^{−9}$ μM CO$_{2}$/CFU for P. aureofaciens and more than ∼3.4 × 10$^{−9}$ μM CO$_{2}$/CFU for Rhodococcus sp.). The kinetics of the metabolic CO$_{2}$ isotopic composition was shown to be determined by the difference in the carbon isotopic characteristics of products in the cell. Upon introduction of glucose into the medium (the preparatory stage of the metabolism), the uptake of intracellular $^{13}$C-depleted products (lipids) is noted; at the stage of the maximal cell growth rate, introduced glucose is mainly metabolized; and at the final stage, upon exhaustion of substrate, the “stored” products—the lipid fraction—get involved in the metabolism. At the maximal rate of glucose uptake, the CO$_{2}$ carbon isotopic fractionation coefficient relative to organic products of microbial biosynthesis was determined to be α = 1.009 ± 0.002.
Starting Page	280
Ending Page	289
Page Count	10
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	82
Issue Number	3
e-ISSN	16083237
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2013-06-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	pseudomonads rhodococci glucose bacterial growth rate respiratory quotient carbon isotope fractionation Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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