NDLI: Structural and functional features of methanotrophs from hypersaline and alkaline lakes

Content Provider	Springer Nature Link
Author	Khmelenina, V. N. Shchukin, V. N. Reshetnikov, A. S. Mustakhimov, I. I. Suzina, N. E. Eshinimaev, B. Ts. Trotsenko, Yu. A.
Copyright Year	2010
Abstract	Ten strains of aerobic methanotrophic bacteria represented by halophilic neutrophiles or halotolerant alkaliphiles were isolated from saline and alkaline lakes of southeast Siberia, Mongolia, Africa, and North America. Based on analysis of the nucleotide sequences of 16S rRNA gene and the pmoA gene encoding particulate methane monooxygenase, the isolates were classified as Methylomicrobium alcaliphilum, Methylomicrobium buryatense, and Methylobacter marinus. All strains of the genus Methylomicrobium were shown to synthesize glycoprotein S-layers located on the cell surface with hexagonal symmetry (p6) as a monolayer of cup-shaped structures or fine “inverted” conical structures and as plates consisting of protein subunits with inclined (p2) symmetry. During adaptation to the high salinity of the medium, isolated methanotrophs synthesize osmoprotectants: ectoine, sucrose, and glutamate. The ectC gene encoding ectoine synthase (EctC) was identified in six methanotrophic strains. Phylogenetic analysis of translated amino acid sequence of the ectC gene fragment suggests lateral transfer of the genes of ectoine synthesis as the most probable way for methanotrophs to acquire resistance to high external salinity.
Starting Page	472
Ending Page	482
Page Count	11
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	79
Issue Number	4
e-ISSN	16083237
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-08-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	halophilic and alkaliphilic methanotrophs taxonomy phylogeny S-layers osmoadaptation ectoine Methylomicrobium Methylobacter Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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