NDLI: Isolation and Comparative Analysis of Glycolipid Fractions in Bifidobacteria

Content Provider	Springer Nature Link
Author	vik, G. I. Astapovich, N. I. Grzegorzewicz, A. Gam'yan, A.
Copyright Year	2005
Abstract	A comparative TLC analysis of lipid extracts from Bifidobacterium longum B 379 M, B. bifidum 791, and B. adolescentis 94 BIM has been performed. It is demonstrated that carbohydrate-containing lipid components were present in the bacteria, which differed in their chromatographic mobility (R $_{ f }$) from similar compounds isolated from actinomycetes Stomatococcus mucilaginosus PCM 2415$^{T}$, Nocardiopsis dassonvillei PCM 2492, Propionibacterium propionicum PCM 2431, Saccharopolyspora hirsuta PCM 2279 (= ATCC 27875$^{T}$), Rhodococcus equi PCM$^{T}$ 559 (= ATCC 3969), and Gordonia bronchialis PCM 2167. Polar lipids of bifidobacteria exhibited the closest similarity to their counterparts from propionic acid bacteria. Preparative chromatography (silica gel column I; elution with chloroform, acetone, and methanol) of the lipid extract of B. adolescentis 94 BIM made it possible to isolate fractions containing nonpolar lipids, glycolipids, and phospholipids. Further purification of the glycolipid fraction (column II; eluant, methanol gradient in chloroform) produced preparations of glycolipids and phospholipids. The preparations were studied by two-dimensional TLC using solvent systems chloroform-methanol-H$_{2}$O MiLi Q (65 : 25 : 4, v/v/v) and n-butanol-acetic acid-H$_{2}$O MiLi Q (60 : 20 : 20, v/v/v) for directions I and II, respectively. Two major glycolipids were revealed (G$_{1}$ and G$_{2}$), in addition to compounds characteristic of the polar lipid group and minor glycolipids (g), the latter being present in considerably lesser amounts.
Starting Page	670
Ending Page	677
Page Count	8
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	74
Issue Number	6
e-ISSN	16083237
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2005-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	actinomycetes bifidobacteria glycolipids chemotaxonomic markers Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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