NDLI: $^{1}$H and $^{31}$P magnetic resonance spectroscopy in a rat model of chronic hepatic encephalopathy: in vivo longitudinal measurements of brain energy metabolism

Content Provider	Springer Nature Link
Author	Rackayova, Veronika Braissant, Olivier McLin, Valérie A. Berset, Corina Lanz, Bernard Cudalbu, Cristina
Copyright Year	2015
Abstract	Chronic liver disease (CLD) leads to a spectrum of neuropsychiatric disorders named hepatic encephalopathy (HE). Even though brain energy metabolism is believed to be altered in chronic HE, few studies have explored energy metabolism in CLD-induced HE, and their findings were inconsistent. The aim of this study was to characterize for the first time in vivo and longitudinally brain metabolic changes in a rat model of CLD-induced HE with a focus on energy metabolism, using the methodological advantages of high field proton and phosphorus Magnetic Resonance Spectroscopy ($^{1}$H- and $^{31}$P-MRS). Wistar rats were bile duct ligated (BDL) and studied before BDL and at post-operative weeks 4 and 8. Glutamine increased linearly over time (+146 %) together with plasma ammonium (+159 %). As a compensatory effect, other brain osmolytes decreased: myo-inositol (-36 %), followed by total choline and creatine. A decrease in the neurotransmitters glutamate (-17 %) and aspartate (-28 %) was measured only at week 8, while no significant changes were observed for lactate and phosphocreatine. Among the other energy metabolites measured by $^{31}$P-MRS, we observed a non-significant decrease in ATP together with a significant decrease in ADP (-28 %), but only at week 8 after ligation. Finally, brain glutamine showed the strongest correlations with changes in other brain metabolites, indicating its importance in type C HE. In conclusion, mild alterations in some metabolites involved in energy metabolism were observed but only at the end stage of the disease when edema and neurological changes are already present. Therefore, our data indicate that impaired energy metabolism is not one of the major causes of early HE symptoms in the established model of type C HE.
Starting Page	1303
Ending Page	1314
Page Count	12
File Format	PDF
ISSN	08857490
Journal	Metabolic Brain Disease
Volume Number	31
Issue Number	6
e-ISSN	15737365
Language	English
Publisher	Springer US
Publisher Date	2015-08-09
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Type C hepatic encephalopathy Bile duct ligation Proton magnetic resonance spectroscopy Phosphorus magnetic resonance spectroscopy Energy metabolism in vivo Neurosciences Neurology Metabolic Diseases Biochemistry Oncology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Neurology (clinical) Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

In vivo studies of brain metabolism in animal models of Hepatic Encephalopathy using $^{1}$H Magnetic Resonance Spectroscopy

Erythropoietin ameliorates the motor and cognitive function impairments in a rat model of hepatic cirrhosis

Impairment of short term memory in rats with hepatic encephalopathy due to bile duct ligation

Intracellular pH measurements of the whole head and the basal ganglia in chronic liver disease: A phosphorus-31 MR spectroscopy study

Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

Metabolic fate of isoleucine in a rat model of hepatic encephalopathy and in cultured neural cells exposed to ammonia

Animal Models in the Study of Episodic Hepatic Encephalopathy in Cirrhosis

Urinary metabolic profiling by $^{1}$H NMR spectroscopy in patients with cirrhosis may discriminate overt but not covert hepatic encephalopathy

Rats with Mild Bile Duct Ligation Show Hepatic Encephalopathy with Cognitive and Motor Impairment in the Absence of Cirrhosis: Effects of Alcohol Ingestion

$^{1}$H and $^{31}$P magnetic resonance spectroscopy in a rat model of chronic hepatic encephalopathy: in vivo longitudinal measurements of brain energy metabolism

Similar Documents

In vivo studies of brain metabolism in animal models of Hepatic Encephalopathy using $^{1}$H Magnetic Resonance Spectroscopy

Erythropoietin ameliorates the motor and cognitive function impairments in a rat model of hepatic cirrhosis

Impairment of short term memory in rats with hepatic encephalopathy due to bile duct ligation

Intracellular pH measurements of the whole head and the basal ganglia in chronic liver disease: A phosphorus-31 MR spectroscopy study

Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

Metabolic fate of isoleucine in a rat model of hepatic encephalopathy and in cultured neural cells exposed to ammonia

Animal Models in the Study of Episodic Hepatic Encephalopathy in Cirrhosis

Urinary metabolic profiling by $^{1}$H NMR spectroscopy in patients with cirrhosis may discriminate overt but not covert hepatic encephalopathy

Rats with Mild Bile Duct Ligation Show Hepatic Encephalopathy with Cognitive and Motor Impairment in the Absence of Cirrhosis: Effects of Alcohol Ingestion

$^{1}$H and $^{31}$P magnetic resonance spectroscopy in a rat model of chronic hepatic encephalopathy: in vivo longitudinal measurements of brain energy metabolism