NDLI: Nonlinear determination of Michaelis-Menten kinetics with model evaluation through estimation of uncertainties

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Nonlinear determination of Michaelis-Menten kinetics with model evaluation through estimation of uncertainties

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Metabolic Brain Disease : Volume 15, Issue 1, March 2000

Metabolic Brain Disease : Volume 14

Metabolic Brain Disease : Volume 13

Metabolic Brain Disease : Volume 12

Nonlinear determination of Michaelis-Menten kinetics with model evaluation through estimation of uncertainties

Content Provider	Springer Nature Link
Author	Mason, Graeme F. Lai, James C. K.
Copyright Year	2000
Abstract	A structured analytic approach was developed to evaluate enzyme kinetic parameters using non-linear least-squares fitting. The approach was implemented in a software package called KinSim and designed to run on Windows 95, 98, and NT platforms. The software and the theoretical approach were tested using kinetic data obtained using citrate synthase with acetyl CoA as a substrate and octanoyl CoA as an inhibitor. Using the software, the data were evaluated for statistical certainty of the presence of competitive and non-competitive inhibition of the enzyme. Given the determination of the presence of both components of inhibition in the experimental setup, the software was used to determine the inhibitory kinetics from the experimental data, including non-normal distributions of uncertainty. Finally, the software was used to evaluate the sensitivities of the experiment design at each concentration of substrate used. The theoretical approach as implemented in the user-friendly software allows investigators to use a structured procedure for evaluating and planning enzyme kinetic and related metabolic studies.
Starting Page	133
Ending Page	149
Page Count	17
File Format	PDF
ISSN	08857490
Journal	Metabolic Brain Disease
Volume Number	15
Issue Number	2
e-ISSN	15737365
Language	English
Publisher	Springer US
Publisher Date	2000-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Michaelis-Menten Enzyme Kinetics Citrate Synthase Computer Software Non-Linear Fitting Neurosciences Neurology Biochemistry Oncology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Neurology (clinical) Cellular and Molecular Neuroscience

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