NDLI: VEGF-mediated suppression of cell proliferation and invasion by miR-410 in osteosarcoma

Content Provider	Springer Nature Link
Author	Zhao, Dong Jia, Peng Wang, Wenliang Zhang, Guangling
Copyright Year	2014
Abstract	MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. The aberrant expression of miRNA has become a major focus in cancer research. This study aimed to investigate the importance of miR-410 in the diagnosis and therapy of osteosarcoma (OS). Western blot analysis showed that the expression of VEGF was higher in Saos-2 and MG-63 cells than that in three other OS cell lines. We also found that miR-410 was lowly expressed and inversely correlated with VEGF expression in OS specimens. Over-expression of miR-410 had a greater repression on VEGF expression than other candidate VEGF-targeting miRNAs. Luciferase reporter assay demonstrated that miR-410 directly decreased VEGF expression by targeting its 3′-untranslated region. Further investigation demonstrated the regulation of miR-410 in OS cells via VEGF. In vitro MTT assay, Transwell, and flow cytometry showed that transfection of the miR-410 expression plasmid inhibited cell proliferation and contributed to apoptosis in OS cells. Moreover, restoration of VEGF reversed the effect of miR-410 on OS cells, and upregulated the expression of phosphorylated AKT. Finally, overexpression of miR-410 also showed a negative effect on tumor growth in vivo. Our findings suggest a cooperative relationship between miR-410 and VEGF in OS cell regulation. This information may help researchers to better understand miRNA regulation in cancer and provide a rationale for developing miRNA-based strategies for OS treatment.
Starting Page	87
Ending Page	95
Page Count	9
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	400
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Springer US
Publisher Date	2014-11-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Osteosarcoma miR-410 VEGF Proliferation Invasion Biochemistry Medical Biochemistry Oncology Cardiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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