NDLI: Okadaic acid induces tyrosine phosphorylation of IκBα that mediated by PKR pathway in human osteoblastic MG63 cells

Content Provider	Springer Nature Link
Author	Morimoto, Hiroyuki Ozaki, Akiko Okamura, Hirohiko Yoshida, Kaya Kitamura, Seiichiro Haneji, Tatsuji
Copyright Year	2005
Abstract	Treatment of human osteosarcoma cell line MG63 cells with okadaic acid stimulated phosphorylation of IκBα, as judged from the results of Western blot analysis and a λ protein phosphatase dephosphorylation assay. The stimulated phosphorylation of IκBα was both time- and dose-dependent. The phosphorylation sites of IκBα were taken to be tyrosine residues because the anti-phospho-tyrosine antibody bound to the samples immunoprecipitated with the anti-IκBα antibody. In the cells treated with 100 nM okadaic acid consequential translocation of NF-κB p65 from the cytosol to the nucleus occurred. Double-stranded RNA-dependent protein kinase (PKR) is a player in the cellular antiviral response and is involved in transcriptional stimulation through activation of NF-κB. We investigated the functional relationship between PKR and IκBα phosphorylation by constructing MG63 PKR K/R cells that produced a catalytically inactive mutant PKR. NF-κB p65 was detected in the nucleus of these cells, even in the unstimulated cells. Although IκBα was degraded phosphorylation of eIF-2α, a substrate of PKR, did not occur in the mutant cells treated with okadaic acid. Our results suggest that okadaic acid-induced tyrosine phosphorylation of IκBα was mediated by PKR kinase activity, thus indicating the involvement of this kinase in the control mechanism governing the activation of NF-κB. (Mol Cell Biochem 276: 211–217, 2005)
Starting Page	211
Ending Page	217
Page Count	7
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	276
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	IκBα tyrosine phosphorylation PKR ser/thr phosphatase inhibitor Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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