NDLI: Non-enzymatic phosphorylation of bovine serum albumin by Cr(V) complexes: Role in Cr(VI)-induced phosphorylation and toxicity

Content Provider	Springer Nature Link
Author	Vasant, Chellappa Sankaramanivel, Sundararaj Jana, Mahadevan Rajaram, Rama Ramasami, Thirumalachari
Copyright Year	2005
Abstract	Evidence for the non-enzymatic phosphorylation of bovine serum albumin (BSA) by sodium bis(2-ethyl-2-hydroxybutyrato)oxochromate(V), Na[Cr$^{V}$O(ehba)$_{2}$], 1, sodium bis(2-hydroxy-2-methylbutyrato)oxochromate(V), Na[Cr$^{V}$O(hmba)$_{2}$], 2 and potassium dichromate, K$_{2}$Cr$_{2}$O$_{7}$, 3 in the presence of labeled adenosine-5′-triphosphate (ATP) under conditions of physiological pH is presented. Aggregation and extent of phosphorylation of BSA mediated by 1, 2 or 3 seems to increase with the concentration and time of incubation of the reaction mixture containing all the reactants. The [γ-$^{32}$P] label in ATP is incorporated into aggregates of BSA in the in vitro reaction of the protein with ATP in the presence of 1, 2 or 3. Phosphorylation of BSA by ATP in the absence of 1, 2 or 3 is negligible. Addition of EDTA reverses aggregation of protein and liberates partially the incorporated phosphate label. The stoichiometry of phosphorylation is found to be the highest and is equal to 12.25 mol PO$_{4}$$^{3−}$/mol BSA in the presence of 500 μM of 1, which decreases to 10.56 mol PO$_{4}$$^{3−}$/mol BSA after EDTA treatment. Resistance to the removal of phosphate label by EDTA increases with increase in time of incubation. Dialysis of phosphorylated BSA reverses the incorporated [γ-$^{32}$P] label only partially, indicating the formation of covalent links of phosphate groups to BSA. Evidence for the site of phosphorylation in the reaction mediated by 1, 2 or 3 being hydroxyl side groups of tyrosine and serine/threonine residues has been gained. Based on the results, a possibility that 1, 2 and 3 mimic the function of tyrosine and serine/threonine kinases has been invoked.
Starting Page	153
Ending Page	164
Page Count	12
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	275
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cr(V) complexes Cr(VI) non-enzymatic phosphorylation reactive oxygen species Src-family kinases apoptosis chromium toxicity Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hexavalent-Chromium-Induced Oxidative Stress and the Protective Role of Antioxidants against Cellular Toxicity.

Cr(VI) increases tyrosine phosphorylation through reactive oxygen species-mediated reactions

CoQ10 Deficiency May Indicate Mitochondrial Dysfunction in Cr(VI) Toxicity

Albumin prevents reactive oxygen species-induced mitochondrial damage, autophagy, and apoptosis during serum starvation

Role of reactive oxygen species and Cr(VI) in Ras-mediated signal transduction

Serum deprivation-induced reactive oxygen species production is mediated by Romo1

Involvement of the p38 MAP kinase in Cr(VI)-induced growth arrest and apoptosis

Constitutive Activation of Epidermal Growth Factor Receptor Promotes Tumorigenesis of Cr(VI)-transformed Cells through Decreased Reactive Oxygen Species and Apoptosis Resistance Development

Interactions of Chromium (III) and Chromium (VI) with Bovine Serum Albumin Studied by UV Spectroscopy, Circular Dichroism, and Fluorimetry

Non-enzymatic phosphorylation of bovine serum albumin by Cr(V) complexes: Role in Cr(VI)-induced phosphorylation and toxicity

Similar Documents

Hexavalent-Chromium-Induced Oxidative Stress and the Protective Role of Antioxidants against Cellular Toxicity.

Cr(VI) increases tyrosine phosphorylation through reactive oxygen species-mediated reactions

CoQ10 Deficiency May Indicate Mitochondrial Dysfunction in Cr(VI) Toxicity

Albumin prevents reactive oxygen species-induced mitochondrial damage, autophagy, and apoptosis during serum starvation

Role of reactive oxygen species and Cr(VI) in Ras-mediated signal transduction

Serum deprivation-induced reactive oxygen species production is mediated by Romo1

Involvement of the p38 MAP kinase in Cr(VI)-induced growth arrest and apoptosis

Constitutive Activation of Epidermal Growth Factor Receptor Promotes Tumorigenesis of Cr(VI)-transformed Cells through Decreased Reactive Oxygen Species and Apoptosis Resistance Development

Interactions of Chromium (III) and Chromium (VI) with Bovine Serum Albumin Studied by UV Spectroscopy, Circular Dichroism, and Fluorimetry

Non-enzymatic phosphorylation of bovine serum albumin by Cr(V) complexes: Role in Cr(VI)-induced phosphorylation and toxicity