NDLI: Kinetic characteristics of folate binding to rat renal brush border membrane in chronic alcoholism

Content Provider	Springer Nature Link
Author	Hamid, Abid Kaur, Jyotdeep
Copyright Year	2005
Abstract	The binding of folic acid to the plasma membrane is an important event for its reabsorption and conservation by renal epithelium. We studied [$^{3}$H] folic acid binding to the renal cortical brush border membrane vesicles (BBMV) in rats after 12 weeks of chronic ethanol ingestion. Our results demonstrated that after chronic ethanol intake, the binding of folic acid to the membrane vesicles: (1) was decreased by a mechanism that decreased B$_{max}$ without affecting K$_{d}$, (2) was insensitive to Na$^{+}$ ions in the medium (3) behaved differently to divalent cations in the medium in contrast to control group. However under such conditions there was no significant effect of ethanol on pH optimum of the process. Binding was reduced at pH less than 6 but there was no effect of ethanol on binding in pH range from 6 to 8. Increasing the osmolarity at pH 5.5 had no effect on the binding of folate to BBMV, thus confirming that the observed changes in B$_{max}$ values were due to site-specific binding in the two groups. Moreover, ethanol does not impart its effect on involvement of sulfhydryl group at the binding site of folate transport system. These findings highlight the possible mechanism of perturbed renal conservation of folate during chronic alcoholism. (Mol Cell Biochem xxx: 219–225, 2005)
Starting Page	219
Ending Page	225
Page Count	7
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	280
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	chronic alcoholism folate folate binding protein reabsorption reduced folate carrier renal tubular brush border membrane Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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