NDLI: Identification of transcriptionally regulated genes in response to cellular iron availability in rat hippocampus

Content Provider	Springer Nature Link
Author	Liu, Mei Xiao, De Sheng Qian, Zhong Ming
Copyright Year	2006
Abstract	The present study was attempted to identify transcriptionally regulated genes of the normal neurocytes responsive to iron availability. Postnatal rat hippocampus cells were primarily cultured either under the iron-loaded or depleted conditions. These cultured cells were applied for the generation of subtracted complementary DNA libraries by the suppression subtraction hybridization (SSH) and for the subsequent identification of differentially expressed transcripts by reverse Northern blot. The differentially expressed genes were chosen to perform sequencing, and then some of them were performed by Northern blot analysis for observation of their expression in the hippocampus of rats with the different iron status. The results indicated that five unique transcripts were strong candidates for differential expression in cellular iron repletion, one of them is a novel sequence (Genbank No.AF433878), while 26 unique transcripts were strong candidates for differential expression in cellular iron deprivation, one of them is a novel sequence (Genbank No. AY 912101). The revealed known genes responsive to iron availability were previously unknown to respond to iron availability, or have not been determined in the brain, have not even been currently determined in their physiological and biological functions. Interestingly, the proteins encoded by most of the known genes are either directly pointed to or indirectly associated with the molecules that play important, even key roles in cellular signal transduction and the cell cycle. These findings lead to the important suggestion that the cellular responses to iron availability involve extensive transcriptional regulation and cellular signal transduction. Therefore, iron may serve as a signal, which directly and/or indirectly regulates or modulates cell functions.
Starting Page	139
Ending Page	147
Page Count	9
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	300
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-12-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Iron Hippocampus Regulation Gene Cardiology Oncology Medical Biochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

MRNA differential display identification of vascular smooth muscle early response genes regulated by PDGF

Induction of P-glycoprotein mRNA transcripts by cycloheximide in animal tissues: Evidence that class I Pgp is transcriptionally regulated whereas class II Pgp is post-transcriptionally regulated

Melatonin increases NMDA receptor subunits 2A and 2B concentrations in rat hippocampus

Regulation of MAPK pathways in response to purinergic stimulation of adult rat cardiac myocytes

Structure and regulation of human troponin genes

Tamoxifen enhances interferon-regulated gene expression in breast cancer cells

Characterization and cellular localization of PSG in rat testis

Receptor-Ck-dependent regulation of genes involved in the cell cycle

Protein kinase CK2 in gene control at cell cycle entry

Identification of transcriptionally regulated genes in response to cellular iron availability in rat hippocampus

Similar Documents

MRNA differential display identification of vascular smooth muscle early response genes regulated by PDGF

Induction of P-glycoprotein mRNA transcripts by cycloheximide in animal tissues: Evidence that class I Pgp is transcriptionally regulated whereas class II Pgp is post-transcriptionally regulated

Melatonin increases NMDA receptor subunits 2A and 2B concentrations in rat hippocampus

Regulation of MAPK pathways in response to purinergic stimulation of adult rat cardiac myocytes

Structure and regulation of human troponin genes

Tamoxifen enhances interferon-regulated gene expression in breast cancer cells

Characterization and cellular localization of PSG in rat testis

Receptor-Ck-dependent regulation of genes involved in the cell cycle

Protein kinase CK2 in gene control at cell cycle entry

Identification of transcriptionally regulated genes in response to cellular iron availability in rat hippocampus