NDLI: Hsp70 attenuates hypoxia/reoxygenation-induced activation of poly(ADP-ribose) synthetase in the nucleus of adult rat cardiomyocytes

Content Provider	Springer Nature Link
Author	Tanaka, Kouichi Toga, Wakako Takahashi, Masaya Kawana, Ken ichiro Miyamoto, Yuki Yoshida, Hiroyuki Takeo, Satoshi
Copyright Year	2003
Abstract	Effects of heat shock protein 70 (Hsp70) translocated to nuclear fraction on hypoxia/reoxygenation injury was examined by using adult cardiomyocytes isolated from rats. Cardiomyocytes were exposed to heat shock at 42°C for 15 min (HS group), and then incubated at 37°C for 6–24 h. Hsp70 production increased and the protein translocated from cytosol to nucleus. The maximum level of Hsp70 in the nuclear fraction was observed 12 h after HS. When cardiomyocytes without exposure to HS (nHS group) were subjected to 120 min hypoxia/15 min reoxygenation (Hypo/Reoxy), post-hypoxic cell viability was approximately 25% of the pre-hypoxic value. A rise in poly(ADP-ribose) synthetase (PARS) activity in the nuclear fraction was observed in nHS group, associated with an increase in polyADP-ribosylated protein. In contrast, post-hypoxic cell viability of HS group was approximately 60% of the pre-hypoxic value. Hypo/reoxy-induced rise in PARS activity and increase in polyADP-ribosylated protein were attenuated in HS group. To confirm the relationship between an increase in cell viability after Hypo/Reoxy and attenuation of PARS activation, cardiomyocytes without exposure to HS were subjected to Hypo/Reoxy in the presence of 1 mM 3-aminobenzamide, an inhibitor of PARS. Treatment of cells with 3-aminobenzamide attenuated Hypo/Reoxy-induced decrease in cell viability. These results suggest that Hsp70 translocated into nucleus after HS may attenuate PARS activation during Hypo/Reoxy, leading to the cytoprotection of cardiomyocytes against Hypo/Reoxy injury.
Starting Page	149
Ending Page	155
Page Count	7
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	248
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2003-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Poly(ADP-ribosyl)ation of proteins and germ cell development in hyperthyroid rat testes

Regulatory mechanisms of poly(ADP-ribose) polymerase

Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation

Molecular heterogeneity and regulation of poly(ADP-ribose) glycohydrolase

Poly (ADP-ribose) polymerase plays an important role in intermittent hypoxia-induced cell death in rat cerebellar granule cells

Small interference RNA against PTP-1B reduces hypoxia/reoxygenation induced apoptosis of rat cardiomyocytes

Functional analysis of poly(ADP-ribose) polymerase in Drosophila melanogaster

Mitochondrial involvement in IGF-1 induced protection of cardiomyocytes against hypoxia/reoxygenation injury

Involvement of oxygen free radicals in the respiratory uncoupling induced by free calcium and ADP-magnesium in isolated cardiac mitochondria: Comparing reoxygenation in cultured cardiomyocytes

Hsp70 attenuates hypoxia/reoxygenation-induced activation of poly(ADP-ribose) synthetase in the nucleus of adult rat cardiomyocytes

Similar Documents

Poly(ADP-ribosyl)ation of proteins and germ cell development in hyperthyroid rat testes

Regulatory mechanisms of poly(ADP-ribose) polymerase

Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation

Molecular heterogeneity and regulation of poly(ADP-ribose) glycohydrolase

Poly (ADP-ribose) polymerase plays an important role in intermittent hypoxia-induced cell death in rat cerebellar granule cells

Small interference RNA against PTP-1B reduces hypoxia/reoxygenation induced apoptosis of rat cardiomyocytes

Functional analysis of poly(ADP-ribose) polymerase in Drosophila melanogaster

Mitochondrial involvement in IGF-1 induced protection of cardiomyocytes against hypoxia/reoxygenation injury

Involvement of oxygen free radicals in the respiratory uncoupling induced by free calcium and ADP-magnesium in isolated cardiac mitochondria: Comparing reoxygenation in cultured cardiomyocytes

Hsp70 attenuates hypoxia/reoxygenation-induced activation of poly(ADP-ribose) synthetase in the nucleus of adult rat cardiomyocytes