NDLI: Interleukin-1β enhances the intracellular accumulation of cholesterol by up-regulating the expression of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase in podocytes

Content Provider	Springer Nature Link
Author	Zhang, Gaofu Li, Qiu Wang, Li Chen, Yaxi Wang, Lijia Zhang, Wei
Copyright Year	2010
Abstract	The aim of this article is to investigate whether interleukin-1β (IL-1β) could regulate the intracellular accumulation of cholesterol and the expression of lipid-metabolism-related regulators in podocytes in vitro and the potential mechanisms. Podocytes were treated with 200 μg/ml of low-density protein (LDL), 20 ng/ml of IL-1β, or 200 μg/ml of LDL plus 5–20 ng/ml of IL-1β for 24 h in vitro. The contents of intracellular cholesterol were determined by enzymatic assays and Oil Red O staining. The levels of LDL receptor (LDLr), 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, sterol regulatory element binding protein 2 (SREBP-2), SREBP cleavage activating protein (SCAP), and insulin-induced gene-1 (Insig-1) expression were characterized by real-time polymerase chain reaction (RT-PCR) and Western blot assays. Treatment with IL-1β or LDL alone increased the contents of intracellular cholesterol (P < 0.05 for both) and lipid droplets, and treatment with both IL-1β and LDL further increased the accumulation of intracellular cholesterol in podocytes (P < 0.05 vs. LDL alone). While loading with LDL significantly inhibited the expression of LDLr, HMG-CoA reductase, nuclear SREBP-2 (nSREBP-2), SCAP, and Insig-1 by 40–62% treatment with IL-1β enhanced the expression of LDLr, HMG-CoA reductase and nSREBP-2, but not Insig-1, in podocytes (P < 0.05 vs. control). Treatment with both LDL and IL-1β significantly increased the levels of LDLr and HMG-CoA reductase expression and the ratio of SCAP to Insig-1, as compared with that in the LDL-treated podocytes (P < 0.05 vs. LDL alone). Our data indicated that IL-1β mitigated the LDL-triggered SCAP-SREBP-2-mediated feedback inhibition on the expression of LDLr and HMG-CoA reductase, leading to the intracellular accumulation of LDL-cholesterol in podocytes in vitro.
Starting Page	197
Ending Page	204
Page Count	8
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	346
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Springer US
Publisher Date	2010-10-10
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Podocyte Lipids accumulation Interleukin-1β Low-density lipoprotein receptor 3-Hydroxy-3-methylglutaryl coenzyme A reductase Cardiology Oncology Medical Biochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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