NDLI: Transcriptional regulation of Na$^{+}$/H$^{+}$ exchanger expression in the intact mouse

Content Provider	Springer Nature Link
Author	Rieder, Carmen V. Fliegel, Larry
Copyright Year	2003
Abstract	We examined regulation of the Na$^{+}$/H$^{+}$ exchanger (NHE1 isoform) in the developing mouse. We generated transgenic mice with the Na$^{+}$/H$^{+}$ exchanger promoter directing expression of the β-Galactosidase reporter. We found that expression of the Na$^{+}$/H$^{+}$ exchanger was maximum in the heart and liver of 12-day-old embryonic mice. Similar results were found in mice using the green fluorescent protein reporter driven by the Na$^{+}$/H$^{+}$ exchanger promoter. Detailed examination of the myocardium revealed that the GFP reporter protein was expressed in the cytoplasm of cardiomyocyte cells. We examined NHE1 protein expression in transgenic mice lacking the transcription factors AP-2α or the transcription factor COUP-TF1. Eighteen-day-old AP-2α heterozygote mice show no large changes in NHE1 expression in heart, lung, liver, kidney and brain. In contrast, 18-day-old embryos from AP-2α null mice showed a large increase in Na$^{+}$/H$^{+}$ exchanger protein expression in the brain. NHE1 protein levels in COUP-TF1 knockout embryos did not differ from wild type embryos. The results suggest that AP-2α and COUP-TF1 are not critical to NHE1 expression in the late stage embryo and that other related transcription factors may function in regulation of the Na$^{+}$/H$^{+}$ exchanger.
Starting Page	87
Ending Page	95
Page Count	9
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	243
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2003-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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