NDLI: Cadmium‐induced apoptosis and phenotypic changes in mouse thymocytes

Content Provider	Springer Nature Link
Author	Dong, Suyun Shen, Han‐Ming Ong, Choon‐Nam
Copyright Year	2001
Abstract	At present cadmium (Cd)‐induced immunotoxicity and the mechanisms involved have not been fully elucidated. The main objective of the present study is to explore the apoptogenic property of Cd in primary cultured mouse thymocytes and its effect on cell surface marker expression and phenotypic changes. Cd‐induced thymocyte apoptosis was determined by TdT‐mediated dUTP nick end labeling (TUNEL) assay, DNA content/cell cycle analysis and DNA gel electrophoresis. The results showed that Cd was able to cause apoptosis in mouse thymocytes in a time‐ and dose‐dependent manner. Moreover, different subsets of thymocytes possessed different susceptibility to the apoptotic effect of Cd, in the order of CD8$^{+}$ > CD4$^{−}$CD8$^{−}$ (double negative cells, DN) > CD4$^{+}$CD8$^{+}$ (double positive cells, DP) > CD4$^{+}$. Cd treatment also altered thymocyte surface marker expression, leading to evident phenotypic changes. Such changes were characterized by a decline in DP cells and a marked decrease in CD4$^{+}$/CD8$^{+}$ ratio, mainly due to a significant increase in CD8$^{+}$ subsets. These observations help to obtain a better understanding of the immunotoxic and immunomodulatory effects of Cd.
Starting Page	11
Ending Page	20
Page Count	10
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	222
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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