NDLI: Differential expression changes in K562 cells during the hemin-induced erythroid differentiation and the phorbol myristate acetate (PMA)-induced megakaryocytic differentiation

Content Provider	Springer Nature Link
Author	Huo, Xiao Fang Yu, Jia Peng, Han Du, Zhan Wen Liu, Xiao Ling Ma, Yan Ni Zhang, Xin Zhang, Yao Zhao, Hua Lu Zhang, Jun Wu
Copyright Year	2006
Abstract	K562 cell line has been used as a model of common progenitor of erythroblasts and magakaryocytes and can be differentiated into erythroid and megakaryocytic lineages by hemin and phorbol myristate acetate (PMA) respectively. We analyzed mRNA expression in un-induced, hemin-induced and PMA-induced K562 cells by differential display reverse transcription polymerase chain reaction (DDRT-PCR) method. 314 differential expression sequence tags (ESTs) were obtained. Among them, 201 ESTs displayed up-regulation and 85 ESTs down-regulation after hemin induction, 186 ESTs showed up-regulation and 72 ESTs down-regulation after PMA induction. The differentially expressed genes included those encoding transcription factors, signaling factors, apoptosis-associated factors and others. 45 of these ESTs stand for genes whose open reading frames were found but whose functions remain unknown. 4 ESTs represent possibly new genes. Furthermore we compared differences of gene expression during hemin-induced erythroid differentiation and PMA-induced megakaryocytic differentiation and found that the expressional changes of some transcription factors and metabolism proteins are the common but the expressional changes of some signal pathways in these two differentiation processes are different. These results suggested that erythroid differentiation and megakaryocytic differentiation are associated in activation and repression of different signal pathways.
Starting Page	155
Ending Page	167
Page Count	13
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	292
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-06-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	K562 cells erythroid differentiation megakaryocytic differentiation DDRT-PCR signaling pathways Cardiology Oncology Medical Biochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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