NDLI: Increase in GP IIb-IIIa and P2Y12 receptors in activated platelets as the possible indicator of de novo protein synthesis

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Increase in GP IIb-IIIa and P2Y12 receptors in activated platelets as the possible indicator of de novo protein synthesis

Content Provider	Springer Nature Link
Author	Sirotkina, O. V. Laskovets, A. B. Andoskin, P. A. Emelyav, A. K. Zabotina, A. M. Vavilova, T. V.
Copyright Year	2016
Abstract	Although platelets lack nuclei, they are capable of de novo protein synthesis. We speculate that key platelet receptors are involved in the regulation of this process, and the changes in their number indicate the de novo protein synthesis in platelets. The object of our study was native platelets obtained from healthy donors. Using flow cytometry and Western blot, we determined the number of GP IIb-IIIa receptors (fibrinogen receptor) and P2Y12 receptors (ADP receptor) on the surface of platelets upon their activation with ADP and collagen. To verify the approaches and techniques used, we studied IL-1β protein, which was previously shown to be synthesized de novo in activated platelets. GP IIb-IIIa receptor numbers correlate with the number of P2Y12 receptors on the cell surface (R = 0.45, p = 0.03). It was demonstrated that the platelet receptor numbers are higher on the surface of the cells with high functional activity. According to the data obtained by Western blot, upon the cell activation with ADP, the number of GP IIb-IIIa and P2Y12 receptors increases, which may serve as evidence of these proteins being synthesized in the activated platelets. It was observed that the level of P2Y12 and IL-1β was lower in the samples where GP IIb-IIIa receptor was blocked by the selective inhibitor, i.e., the Fab fragment of the antibodies that specifically recognizes the GP IIb-IIIa complex. This suggests the important role of GP IIb-IIIa receptor in the regulation of protein synthesis.
Starting Page	111
Ending Page	117
Page Count	7
File Format	PDF
ISSN	00268933
Journal	Molecular Biology
Volume Number	50
Issue Number	1
e-ISSN	16083245
Language	English
Publisher	Pleiades Publishing
Publisher Date	2016-03-30
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	de novo protein synthesis platelets in vitro platelet activation platelet receptor GP IIb-IIIa platelet receptor P2Y12 flow cytometry Western blott Life Sciences Biochemistry Human Genetics
Content Type	Text
Resource Type	Article
Subject	Structural Biology Biophysics

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