NDLI: Leucine Zipper Motif Drives the Transmembrane Domain Dimerization of E-cadherin

Content Provider	Springer Nature Link
Author	Xu, Lida Hu, Ting Ting Luo, Shi Zhong
Copyright Year	2013
Abstract	E-cadherin is a transmembrane glycoprotein which is involved in the Ca$^{2+}$-dependent cell–cell adhesion, and the adhesiveness is heavily dependent on the homodimerization of this molecule. Previous studies have shown that both the extracellular domain and cytoplasmic domain of E-cadherin contribute to its homodimerization. However, the roles of the transmembrane(TM) domain in the E-cadherin homodimerization have not been discussed in detail. In our experiments, SDS-PAGE showed higher molecular weight bands for the synthetic E-cadherin TM peptide, which indicated that the E-cadherin TM peptide is able to dimerize in the SDS micelle. The TOXCAT assay proved that the E-cadherin TM domain can form a moderate homo-oligomer in the Escherichia coli inner membrane. Furthermore, mutational analyses using the TOXCAT assays revealed that, instead of the common GxxxG dimerization motif, the leucine zipper motif is essential for the dimerization of the E-cadherin TM domain. Combining our experiment data and the computational simulation results, we provide insights for understanding the roles of the TM domain in the E-cadherin dimerization.
Starting Page	95
Ending Page	102
Page Count	8
File Format	PDF
ISSN	15733149
Journal	International Journal of Peptide Research and Therapeutics
Volume Number	20
Issue Number	1
e-ISSN	15733904
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-09-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dimerization E-cadherin GxxxG motif Leucine zipper motif TOXCAT Transmembrane domain Biochemistry Animal Anatomy / Morphology / Histology Polymer Sciences Pharmaceutical Sciences/Technology Pharmacology/Toxicology Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Drug Discovery Biochemistry Molecular Medicine Bioengineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Immunohistological Detection of Leucine-Enkephalin in the Digestive System of the Scallop Chlamys farreri

Proteome-Wide Inference of Src Homology 3 Domain-Binding Peptides

Synthesis of a Double Transmembrane Domain Fragment of Ste2p by Native Chemical Ligation

Immunohistochemical Study of Leucine-Enkephalin and Delta Opioid Receptor in Mantles and Feet of the Octopus Octopus ocellatus Gray

The Chemical Synthesis of the Collagenous Domain of the Hormone Adiponectin

Modern Solid Phase Peptide Synthesis and its Applications

Drug Development-targeted Screening of Leptin Agonist Glycopeptides

Substitution of the Echistatin Amino Acid Motif RGDD with KGDW Enhances Inhibition of Platelet Aggregation and Thrombogenesis

Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines

Leucine Zipper Motif Drives the Transmembrane Domain Dimerization of E-cadherin

Similar Documents

Immunohistological Detection of Leucine-Enkephalin in the Digestive System of the Scallop Chlamys farreri

Proteome-Wide Inference of Src Homology 3 Domain-Binding Peptides

Synthesis of a Double Transmembrane Domain Fragment of Ste2p by Native Chemical Ligation

Immunohistochemical Study of Leucine-Enkephalin and Delta Opioid Receptor in Mantles and Feet of the Octopus Octopus ocellatus Gray

The Chemical Synthesis of the Collagenous Domain of the Hormone Adiponectin

Modern Solid Phase Peptide Synthesis and its Applications

Drug Development-targeted Screening of Leptin Agonist Glycopeptides

Substitution of the Echistatin Amino Acid Motif RGDD with KGDW Enhances Inhibition of Platelet Aggregation and Thrombogenesis

Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines

Leucine Zipper Motif Drives the Transmembrane Domain Dimerization of E-cadherin