NDLI: Antibacterial effect of different extracts from Wikstroemia indica on Escherichia coli based on microcalorimetry coupled with agar dilution method

Content Provider	Springer Nature Link
Author	Chen, Chang Qu, Fen Wang, Jian Xia, Xinhua Wang, Jiabo Chen, Zhe Ma, Xiao Wei, Shizhang Zhang, Yaming Li, Jianyu Gong, Man Wang, Ruilin Liu, Honghong Yang, Zhirui Li, Yonggang Zhao, Yanling Xiao, Xiaohe
Copyright Year	2015
Abstract	The root of Wikstroemia indica has been widely used in China as folk medicine for the treatment for arthritis, whooping cough, cancer, and bacillosis. However, the constituents which have antibacterial activity were not clarified yet. In this study, the antibacterial effect of five extracts from W. indica on Escherichia coli was evaluated by microcalorimetry coupled with agar dilution method. The ethanol extract of W. indica was isolated with organic solvents of different polarities including petroleum (P.E.) extract, chloroform (CHCl$_{3}$) extract, ethyl acetate (EtOAc) extract, n-butylalcohol (nBuOH) extract, and residue extract. The metabolic profiles of E. coli growth at 37 °C were measured by microcalorimetry. According to the principal component analysis, k $_{1}$, k $_{2}$, and P $_{1}$ were obtained from heat flow power–time (HFP–time) curve. The agar dilution method was performed to verify the results of thermodynamics. The results of microcalorimetric experiment indicated that EtOAc fraction demonstrated the strongest antibacterial activity with half-inhibitory concentration of 92.4 μg mL$^{−1}$. Meanwhile, similar results were gained from the common method of agar diffusion, which suggested that EtOAc extract could be further developed as antibacterial bioactive fraction of W. indica. Altogether, microcalorimetry is a useful technique to provide sufficient quantitative information and evaluate the antimicrobial effect with its sensitive.
Starting Page	1583
Ending Page	1590
Page Count	8
File Format	PDF
ISSN	13886150
Journal	Journal of thermal analysis
Volume Number	123
Issue Number	2
e-ISSN	15882926
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-09-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Wikstroemia indica Anti-E. coli Bioactive fraction Microcalorimetry Agar dilution method Physical Chemistry Analytical Chemistry Polymer Sciences Inorganic Chemistry Measurement Science and Instrumentation
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry Condensed Matter Physics

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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