NDLI: In vitro study of proteins surface activity by tritium probe

Content Provider	Springer Nature Link
Author	Chernysheva, Maria G. Badun, Gennadii A.
Copyright Year	2010
Abstract	A new technique for in vitro studies of biomacromolecules interactions, their adsorption at aqueous/organic liquid interfaces and distribution in the bulk of liquid/liquid systems was developed. The method includes (1) tritium labeling of biomolecules by tritium thermal activation method and (2) scintillation phase step with organic phase, which can be concerned as a model of cellular membrane. Two globular proteins lysozyme and human serum albumin tested. We have determined the conditions of tritium labeling when labeled by-products can be easy separated by means of dialysis and size-exclusion chromatography. Scintillation phase experiments were conducted for three types of organic liquids. Thus, the influences of the nature of organic phase on proteins adsorption and its distribution in the bulk of aqueous/organic liquid system were determined. It was found that proteins possess high surface activity at aqueous/organic liquid interface. Furthermore, values of hydrophobicity of globular proteins were found by the experiment.
Starting Page	835
Ending Page	840
Page Count	6
File Format	PDF
ISSN	02365731
Journal	Journal of Radioanalytical and Nuclear Chemistry
Volume Number	286
Issue Number	3
e-ISSN	15882780
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-08-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tritium labeled macromolecules Scintillation phase method Inorganic Chemistry Diagnostic Radiology Nuclear Physics, Heavy Ions, Hadrons Physical Chemistry Nuclear Chemistry
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Pollution Health, Toxicology and Mutagenesis Radiology, Nuclear Medicine and Imaging Analytical Chemistry Public Health, Environmental and Occupational Health Nuclear Energy and Engineering

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