NDLI: Required polymer lengths per precipitated protein molecule in protein-polymer interaction

Content Provider	Springer Nature Link
Author	Capito, Florian Kolmar, Harald Stanislawski, Bernd Skudas, Romas
Copyright Year	2014
Abstract	Protein precipitation using non-charged and charged polymers is a common method for protein purification, gaining broader interest among manufacturers in downstream processing. While during polymer- surface interactions, the formation of loops, tails and trains has been known for quite a long time, details of polymer conformation and chain length, interacting with the protein during protein precipitation are not fully discovered. Our research presents a more profound understanding of polymer-protein interaction, combining fluorescence and infrared spectroscopic measurements of proteins and polymer standards with well defined chain length to confirm different models of protein-polymer interaction. Lysozyme, chymotrypsinogen A, myoglobin and a monoclonal antibody, all of different molecular weight, isoelectric point and charge distribution at the protein surface, were used for protein-polymer precipitation. The use of polymers of various charge density and chain length showed that the required polymer length per precipitated protein (L$_{def}$) is up to 25-times larger than the diameter of the corresponding protein, depending on the surface charge distribution of the protein, and its isoelectric point, as well as the charge density of the polymer. Our results support proposed mechanisms of polymer wrapping and loop formation for optimal charge neutralization during complexation and imply interaction of several polymer chains per precipitated protein molecule. Electrophoretic light scattering showed a qualitative correlation of the zeta potential of analyzed polymers with their corresponding L$_{def}$ values. Comparing protein precipitation behavior of long and short polymer chains, the latter exhibited reduced precipitation efficiency, visible as elevated L$_{def}$.
Starting Page	1
Ending Page	13
Page Count	13
File Format	PDF
ISSN	10229760
Journal	Journal of Polymer Research
Volume Number	21
Issue Number	2
e-ISSN	15728935
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-01-11
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Protein-polymer interaction Chain length Precipitation conditions Zeta potential Polymer Sciences Industrial Chemistry/Chemical Engineering Characterization and Evaluation of Materials
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Materials Chemistry Polymers and Plastics

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