NDLI: Interaction of Human Serum Albumin with Indomethacin: Spectroscopic and Molecular Modeling Studies

Content Provider	Springer Nature Link
Author	Zhang, Jing Sun, Hui Hui Zhang, Ye Zhong Yang, Li Yun Dai, Jie Liu, Yi
Copyright Year	2012
Abstract	In this work, the interaction between indomethacin (IM) and human serum albumin (HSA) under simulative physiological conditions was investigated by the methods of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and molecular modeling. The experiment results showed that the fluorescence quenching of HSA by IM was a result of the formation of an IM–HSA complex and the corresponding association constants (K $_{a}$) between IM and HSA at four different temperatures were determined according to the modified Stern–Volmer equation. The resulting thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicate that the hydrophobic force plays a major role for IM–HSA association, but hydrogen bonds also could not be excluded. A molecular modeling study further confirmed the binding mode and indicated that the binding of IM to HSA primarily takes place in sub-domain IIA (site I). The conformational investigation showed that the presence of IM decreased the α-helical content of HSA and induced slight unfolding of the polypeptides of protein, which confirmed that some microenvironmental and conformational changes occur for HSA molecules.
Starting Page	422
Ending Page	435
Page Count	14
File Format	PDF
ISSN	00959782
Journal	Journal of Solution Chemistry
Volume Number	41
Issue Number	3
e-ISSN	15728927
Language	English
Publisher	Springer US
Publisher Date	2012-03-09
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Indomethacin Human serum albumin Fluorescence spectrum Binding site Molecular modeling Circular dichroism (CD) Inorganic Chemistry Physical Chemistry Geochemistry Condensed Matter Physics Industrial Chemistry/Chemical Engineering Oceanography
Content Type	Text
Resource Type	Article
Subject	Biochemistry Molecular Biology Biophysics Physical and Theoretical Chemistry

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