NDLI: Preparation and characterization of three-dimensional nanostructured macroporous bacterial cellulose/agarose scaffold for tissue engineering

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Preparation and characterization of three-dimensional nanostructured macroporous bacterial cellulose/agarose scaffold for tissue engineering

Content Provider	Springer Nature Link
Author	Yang, Chunxi Gao, Chuan Wan, Yizao Tang, Tingting Zhang, Shuhong Dai, Kerong
Copyright Year	2010
Abstract	We fabricated a three-dimensional nanostructured macroporous bacterial cellulose scaffold (3D BC scaffold) and a three-dimensional nanostructured macroporous bacterial cellulose/agarose scaffold (3D BC/A). Results of scanning electron microscopy (SEM) and mercury intrusion porosimeter showed that both the 3D BC and the 3D BC/A have interconnected macropores characterized by nanofibrous pore walls (The diameter of the dominant pores was about 100 μm and ranges from <1 μm to >1,000 μm). 3D BC/A also has high surface area (80 ± 5 m$^{2}$/g) and sufficient porosity (88.5 ± 0.4%) compare with 3D BC (surface area: 92.81 ± 2.02 m$^{2}$/g; porosity 90.42 ± 0.24%). 3D BC/A do support C5.18 cell and hBMSC attachment, proliferation evaluated with SEM, confocal microscopy and cell proliferation assay. Furthermore, 3D/ABC has enhanced mechanical property (ultimate compressive strength: 26.26 ± 4.6 kPa, Young’s modulus: 39.26 ± 5.72 kPa)) than that 3D/BC has (ultimate compressive strength: 7.04 ± 2.34 kPa, Young’s modulus: 10.76 ± 3.52 kPa). Overall, the 3D BC/A scaffold had more potential than 3D BC scaffold for using as a scaffold for tissue engineering and tissue repair applications.
Starting Page	545
Ending Page	552
Page Count	8
File Format	PDF
ISSN	13802224
Journal	Journal of Porous Materials
Volume Number	18
Issue Number	5
e-ISSN	15734854
Language	English
Publisher	Springer US
Publisher Date	2010-09-02
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adult human bone marrow derived stromal cells (hBMSCs) Bacterial cellulose Chondroblasts Macropore structure Nano-biomaterials Tissue engineering Physical Chemistry Catalysis Characterization and Evaluation of Materials
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Materials Science Mechanical Engineering

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