NDLI: Microarchitectural and mechanical characterization of chitosan/hydroxyapatite/demineralized bone matrix composite scaffold

Content Provider	Springer Nature Link
Author	Yan, Zhang Ruixin, Li Yubo, Fan Hao, Liu Yong, Guo Liang, Wang Caihong, Shi Dong, Zhu Xizheng, Zhang
Copyright Year	2011
Abstract	Porous degradable scaffolds are used extensively in bone tissue engineering. As well as material type, the architectural and mechanical characterizations of scaffolds are important to facilitate cell and tissue growth. Matrices composed of hydroxyapatite (HA), chitosan (CS) and demineralized bone matrix (DBM) may create an appropriate environment for the regeneration of bones. In this study, CS/HA/DBM scaffolds with sufficient structural integrity and high interconnected porosity were produced using different combinations of CS, HA and DBM. Both mechanical and biological properties of porous scaffolds were determined by local microarchitecture whose parameters were quantified based on micro computed tomography (Micro-CT) analysis. Within porosity range of 48–65%, the ranges of average compressive modulus and ultimate strength of the scaffolds were 3 ± 1–6 ± 1 kPa and 11 ± 2–24 ± 2 kPa, respectively. With the increase of HA concentration at the equal weight of DBM, the average trabecular thickness and trabecular separation increased and bone surface/volume ratio decreased, resulting in higher volume fraction and lower total porosity. In vitro, MC3T3-E1 preosteoblast cells were used to investigate cell attachment, spreading and proliferation on the scaffolds via hematoxyline and eosin (HE), scanning electron microscopy (SEM) and MTS assay. The results showed that MC3T3-E1 cells adhered to the surface of composite scaffolds, cell number increased with culture time. Cell viability increased with the HA particles decreased, changed little with the DBM increased. Consideration of the microarchitectural and mechanical characterization and biocompatibility of the scaffolds, 3:3:1.5 and 3:5:1.5 groups were believed to be the best in our study.
Starting Page	251
Ending Page	259
Page Count	9
File Format	PDF
ISSN	13802224
Journal	Journal of Porous Materials
Volume Number	19
Issue Number	2
e-ISSN	15734854
Language	English
Publisher	Springer US
Publisher Date	2011-04-19
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chitosan/Hydroxyapatite/Demineralized bone matrix Micro computed tomography Mechanical property Characterization and Evaluation of Materials Physical Chemistry Catalysis
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Materials Science Mechanical Engineering

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