NDLI: Locating cryptotephra in lake sediments using fluid imaging technology

Content Provider	Springer Nature Link
Author	D’Anjou, Robert M. Balascio, Nicholas L. Bradley, Raymond S.
Copyright Year	2014
Abstract	We report a new approach to locate and quantify cryptotephra in sedimentary archives using a continuously-imaging Flow Cytometer and Microscope (FlowCAM$^{®}$). The FlowCAM rapidly photographs particles flowing in suspension past a microscope lens and performs semi-automated analysis of particle images. It has had primarily biological applications, although the potential sedimentological applications are numerous. Here we test the ability of this instrument to image irregularly shaped, vesicular glass shards and to screen sediment samples for the presence of cryptotephra. First, reference samples of basalt and rhyolite tephra (sieved <63 μm) were analyzed with the FlowCAM, demonstrating the ability of the instrument to photograph individual tephra shards. The highest-quality images were used to create a reference library of tephra particles, against which other particle morphologies could be automatically compared. Lake sediment samples with known concentrations of tephra were then analyzed. The tephra image library was used to perform pattern recognition calculations, automatically distinguishing tephra-like images from other particles in the sediment samples. The number of tephra shards identified by the FlowCAM technique was compared to manual counting using a polarizing light microscope, demonstrating that this rapid approach is capable of determining the relative concentrations of tephra in a given sediment sample. However, the FlowCAM systematically underestimates tephra concentrations relative to manual counts. We conclude that with a well-developed image library, the FlowCAM can be an effective tool for cryptotephra and sedimentological applications, but it may be inappropriate for large volume samples or if particle morphologies are outside the range of the image library.
Starting Page	257
Ending Page	264
Page Count	8
File Format	PDF
ISSN	09212728
Journal	Journal of Paleolimnology
Volume Number	52
Issue Number	3
e-ISSN	15730417
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-08-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tephra Cryptotephra Lake sediment Fluid imaging Particle recognition FlowCAM Paleontology Sedimentology Climate Change Physical Geography Freshwater & Marine Ecology Geology
Content Type	Text
Resource Type	Article
Subject	Earth-Surface Processes Aquatic Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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