NDLI: The Mechanism of 2,2,2-Trichloroacetic Acid-Induced Protein Precipitation

Content Provider	Springer Nature Link
Author	Sivaraman, T. Kumar, T. K. S. Jayaraman, G. Yu, C.
Copyright Year	1997
Abstract	The mechanism of 2,2,2-trichloroacetic acid (TCA)-induced precipitation of proteins is studied. The TCA-induced protein precipitation curves are observed to be U-shaped. It is bound that the protein-precipitate-inducing effects of TCA are due to the three chloro groups in the molecule. Using cardiotoxin III (CTX III) isolated from the Taiwan cobra (Naja naja atra), as a model protein, we attempt to understand the molecular basis for the TCA-induced effects. Employing circular dichroism, proton–deuterium exchange in conjunction with conventional 2D NMR techniques, and 1-anilino naphthalene-8-sulfonate-binding experiments, we demonstrate that CTX III is in a partially structured state similar to the ‘A state’ in 3% w/v TCA. It is postulated that the formation of this ‘sticky’ partial structured ‘A state’ in the TCA-induced unfolding pathway is responsible for the acid-induced protein precipitation.
Starting Page	291
Ending Page	297
Page Count	7
File Format	PDF
ISSN	02778033
Journal	The Protein Journal
Volume Number	16
Issue Number	4
e-ISSN	15734943
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Organic Chemistry Bioorganic Chemistry Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Biochemistry

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