NDLI: Effect of Glucose on the Lactoferrin’s Conformation and its Effect on MC 3T3-E1 Cell Proliferation

Content Provider	Springer Nature Link
Author	Wen, Pengcheng Guo, Huiyuan Zhang, Hao Gan, Bozhong Ding, Qingbo Ren, Fazheng
Copyright Year	2012
Abstract	The objective of this research was to investigate the influence of glucose on bovine lactoferrin’s (LF) conformation, thermodynamic stability and osteoblastic cell proliferation. The conformation and thermodynamic stability of LF was detected by spectroscopic and differential scanning calorimetry (DSC). The osteoblastic cell proliferation of LF at physiological concentrations (100 μg/ml) was measured by BrdU incorporation. The binding constant between glucose and LF is KSV = 5 × 10$^{−3}$, and Tyr residues of LF were located in a more hydrophobic environment, while Trp residues were located in a more hydrophilic environment. LF with glucose had increased α-helix and β-sheet contents by 6 and 14 %, respectively. It showed a two-step denaturation of LF. There was a gradual changs in the denaturation temperature and the calorimetric enthalpies (ΔHcal) with a growing concentration of glucose. It has also revealed that glucose dose dependently reduced the ability of LF to increase MC 3T3-E1 cell proliferation. Increasing the binding with glucose, LF might cause to change its native state, which reduced the stimulation activity of osteoblasts cell proliferation.
Starting Page	300
Ending Page	305
Page Count	6
File Format	PDF
ISSN	15723887
Journal	The Protein Journal
Volume Number	31
Issue Number	4
e-ISSN	15734943
Language	English
Publisher	Springer US
Publisher Date	2012-03-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lactoferrin Glucose Fluorescence Spectroscopy CD DSC MC3T3-E1 Animal Anatomy / Morphology / Histology Organic Chemistry Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Bioengineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effect of glucose on the lactoferrin's conformation and its effect on MC 3T3-E1 cell proliferation.

Molecular Characterisation of Group IVA (Cytosolic) Phospholipase A$_{2}$ in Murine Osteoblastic MC3T3-E1 Cells

Calprotectin Pegylation Enhanced Its Physical and Structural Properties

Effect of Heat and pH Denaturation on the Structure and Conformation of Recombinant Human Hepatic Stimulator Substance

Comparison of Guanidine Hydrochloride (GdnHCl) and Urea Denaturation on Inactivation and Unfolding of Human Placental Cystatin (HPC)

Binding of Al(III)-tetracarboxyphthalocyanine to Hemoglobin and Myoglobin

Effect of Glycation of Hemoglobin on its Interaction with Trifluoperazine

Monitoring the Active Conformation of Green Fluorescent Protein (GFP) and β-Glucosidase Adsorbed on Soil Particles

Effect of Chemical Chaperones on Glucose-Induced Lysozyme Modifications

Effect of Glucose on the Lactoferrin’s Conformation and its Effect on MC 3T3-E1 Cell Proliferation

Similar Documents

Effect of glucose on the lactoferrin's conformation and its effect on MC 3T3-E1 cell proliferation.

Molecular Characterisation of Group IVA (Cytosolic) Phospholipase A$_{2}$ in Murine Osteoblastic MC3T3-E1 Cells

Calprotectin Pegylation Enhanced Its Physical and Structural Properties

Effect of Heat and pH Denaturation on the Structure and Conformation of Recombinant Human Hepatic Stimulator Substance

Comparison of Guanidine Hydrochloride (GdnHCl) and Urea Denaturation on Inactivation and Unfolding of Human Placental Cystatin (HPC)

Binding of Al(III)-tetracarboxyphthalocyanine to Hemoglobin and Myoglobin

Effect of Glycation of Hemoglobin on its Interaction with Trifluoperazine

Monitoring the Active Conformation of Green Fluorescent Protein (GFP) and β-Glucosidase Adsorbed on Soil Particles

Effect of Chemical Chaperones on Glucose-Induced Lysozyme Modifications

Effect of Glucose on the Lactoferrin’s Conformation and its Effect on MC 3T3-E1 Cell Proliferation