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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kawasaki, Takayasu Fujioka, Jun Imai, Takayuki Tsukiyama, Koichi |
| Copyright Year | 2012 |
| Abstract | Aggregation of lysozyme in an acidic solution generates inactive amyloid-like fibrils, with a broad infrared peak appearing at 1,610–1,630 cm$^{−1}$, characteristic of a β-sheet rich structure. We report here that spontaneous refolding of these fibrils in water could be promoted by mid-infrared free-electron laser (mid-IR FEL) irradiation targeting the amide bands. The Fourier transform infrared spectrum of the fibrils reflected a β-sheet content that was as low as that of the native structure, following FEL irradiation at 1,620 cm$^{−1}$ (amide I band); both transmission-electron microscopy imaging and Congo Red assay results also demonstrated a reduced fibril structure, and the enzymatic activity of lysozyme fibrils recovered to 70–90 % of the native form. Both irradiations at 1,535 cm$^{−1}$(amide II band) and 1,240 cm$^{−1}$ (amide III band) were also more effective for the refolding of the fibrils than mere heating in the absence of FEL. On the contrary, either irradiation at 1,100 or 2,000 cm$^{−1}$ afforded only about 60 % recovery of lysozyme activity. These results indicate that the specific FEL irradiation tuned to amide bands is efficient in refolding of lysozyme fibrils into native form. |
| Starting Page | 710 |
| Ending Page | 716 |
| Page Count | 7 |
| File Format | |
| ISSN | 15723887 |
| Journal | The Protein Journal |
| Volume Number | 31 |
| Issue Number | 8 |
| e-ISSN | 15734943 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2012-10-11 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aggregation Amide bands Amyloid fibril Free electron laser Refolding Biochemistry Bioorganic Chemistry Organic Chemistry Animal Anatomy / Morphology / Histology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Bioengineering |
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