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| Content Provider | Springer Nature Link |
|---|---|
| Author | Qian, Minxie Huang, Qichen Wu, Guangteng Lai, Luhua Tang, Youqi Pei, Jianfeng Kusuki, Masami |
| Copyright Year | 2011 |
| Abstract | The structure of acetamidase/formamidase (Amds/Fmds) from the archaeon Thermoanaerobacter tengcongensis has been determined by X-ray diffraction analysis using MAD data in a crystal of space group P2$_{1}$, with unit-cell parameters a = 41.23 (3), b = 152.88 (6), c = 100.26 (7) Å, β = 99.49 (3) ° and been refined to a crystallographic R-factor of 17.4% and R-free of 23.7%. It contains two dimers in one asymmetric unit, in which native Amds/Fmds (TE19) contains of the 32 kDa native protein. The final model consists of 4 monomer (299 amino acids residues with additional 2 expression tag amino acids residues), 5 Ca$^{2+}$, 4 Zn$^{2+}$ and 853 water molecules. The monomer is composed by the following: an N-domain which is featuring by three-layers β/β/β; a prominent excursion between N-terminal end of strand β$_{7}$ and β$_{11}$, which contains four-stranded antiparallel β sheet; an C-domain which is formed by the last 82 amino acid residues with the feature of mixed α/β structure. The protein contains ion-pair Ca$^{2+}$–Zn$^{2+}$. The portion of three-layer β/β/β along with the loops provides four protein ligands to the tightly bound Ca$^{2+}$, three water molecules complete the coordination; and provides five protein ligands to the tightly bound Zn$^{2+}$, one water molecule complete the coordination. |
| Starting Page | 166 |
| Ending Page | 174 |
| Page Count | 9 |
| File Format | |
| ISSN | 15723887 |
| Journal | The Protein Journal |
| Volume Number | 31 |
| Issue Number | 2 |
| e-ISSN | 15734943 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2011-12-30 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Acetamidase/formamidase Structural genomics Crystal structure Organic Chemistry Biochemistry Animal Anatomy / Morphology / Histology Bioorganic Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Bioengineering |
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