NDLI: The Orientation of the Phosphorescence Dipole Moment of Erythrosine B Within Its Molecular Frame

Content Provider	Springer Nature Link
Author	Lettinga, M. P. Klarenbeek, E. M. Zuilhof, Han van Zandvoort, M. A. M. J.
Copyright Year	1999
Abstract	For an unequivocal interpretation of time-resolved phosphorescence depolarization measurements on dye molecules in biophysical systems, the orientation of the transition dipole moments within the frame of the dye has to be known. The goal of this work is to find the orientation of the phosphorescence dipole moment of erythrosine B within its molecular frame. To this end, we first determine four difference angles, exciting a molecule in two of its absorption bands and measuring the fluorescence and phosphorescence anisotropy for each excitation wavelength. The next step is to position the transition dipole moments within the molecular frame, combining the results of angle-resolved fluorescence depolarization measurements with the measured difference angles. It is shown that the phosphorescence dipole moment is tilted out of the plane of the molecule. Additionally, we find that the phosphorescence dipole moment is located above the fluorescence dipole moment and not above the absorption dipole moment. This induces an extra difference angle between the absorption and the phosphorescence dipole moments which in the past has been interpreted erroneously as a larger tilt out of the plane of the molecule.
Starting Page	265
Ending Page	279
Page Count	15
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	9
Issue Number	3
e-ISSN	15734994
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Analytical Chemistry Biochemistry Biophysics/Biomedical Physics
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Phosphorescence of 8-Azasteroid in Solid Solution

Ab Initio Study of the Phosphorescence of Nitrite Ions

Phosphorescence of Enaminodicarbonyl-8-Azasteroids and Related Compounds

Analytical Possibilities of the Fine-Structure Phosphorescence Spectrometry Technique for Registration of Polychlorinated Dioxins

Is Phosphorescence Lifetime an Indicator of Angiogenesis in Cortical Sarcoma?

Dipole Moments of 4′-Aminoflavonol Fluorescent Probes in Different Solvents

Saturation effects on phosphorescence anisotropy measurements at high laser pulse energies

Emerging Applications of Phosphorescent Metalloporphyrins

Editorial: Plasmonics

The Orientation of the Phosphorescence Dipole Moment of Erythrosine B Within Its Molecular Frame

Similar Documents

Phosphorescence of 8-Azasteroid in Solid Solution

Ab Initio Study of the Phosphorescence of Nitrite Ions

Phosphorescence of Enaminodicarbonyl-8-Azasteroids and Related Compounds

Analytical Possibilities of the Fine-Structure Phosphorescence Spectrometry Technique for Registration of Polychlorinated Dioxins

Is Phosphorescence Lifetime an Indicator of Angiogenesis in Cortical Sarcoma?

Dipole Moments of 4′-Aminoflavonol Fluorescent Probes in Different Solvents

Saturation effects on phosphorescence anisotropy measurements at high laser pulse energies

Emerging Applications of Phosphorescent Metalloporphyrins

Editorial: Plasmonics

The Orientation of the Phosphorescence Dipole Moment of Erythrosine B Within Its Molecular Frame