NDLI: Two New Mononuclear Copper(II)-Dipeptide Complexes of 2-(2′-Pyridyl)Benzoxazole: DNA Interaction, Antioxidation and in Vitro Cytotoxicity Studies

Content Provider	Springer Nature Link
Author	Gan, Qian Qi, Yongyu Xiong, Yahong Fu, Yinlian Le, Xueyi
Copyright Year	2016
Abstract	Two new mononuclear mixed ligand copper(II) complexes [Cu(PBO)(Gly-gly)(H$_{2}$O)]·ClO$_{4}$·1.5H$_{2}$O (1) and [Cu(PBO)(Gly-L-leu)(H$_{2}$O)]·ClO$_{4}$ (2) (PBO is 2-(2′-pyridyl)benzoxazole, Gly-gly and Gly-L-leu are Glycyl-glycine anion and Glycyl-L-leucine anion, respectively), have been prepared and characterized by various analytical and spectral techniques. The interactions of the complexes with DNA were investigated using multi-spectroscopic methods (absorption, emission, circular dichroism), viscometry and electrochemical titration as well as molecular docking technique. The results indicated that 1 and 2 are bound to calf thymus DNA (CT-DNA) through an intercalative mode. The thermodynamic analyses revealed that the reactions between the Cu(II) complexes with DNA are spontaneous with negative Gibbs free energy (ΔG). The positive changes of enthalpy (ΔH) and entropy (ΔS) suggested that the binding processes are dominated by hydrophobic interaction accompanying with endothermic. Also, the complexes exhibited efficient oxidative cleavage of pBR322 plasmid DNA in the presence of ascorbic acid, probably induced by •OH as reactive oxygen species. In addition, 1 and 2 displayed excellent antioxidant activities with the IC$_{50}$ values of 0.112 and 0.191 μM, respectively, using the mean of nitroblue tetrazolium (NBT) photochemical reduction under a nonenzymatic condition. Moreover, the complexes were screened for their in vitro cytotoxicity against three human carcinoma cell lines (HeLa, PC-3 and A549), in which 2 owns higher cytotoxicity, which was consistent with DNA binding and cleavage ability order of the complexes. This results showed the in vitro biochemical potentials of the Cu(II)-dipeptide complexes with aromatic heterocyclic, viz. effective metallopeptide-nucleases, SOD mimics and non-platinum chemotherapeutic metallopharmaceuticals and their structure-activity relationship, which may contribute to the rational molecular design of new metallopeptide based chemotherapeutic agents.
Starting Page	701
Ending Page	714
Page Count	14
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	27
Issue Number	2
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2016-12-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cu(II)-dipeptide complexes DNA interaction Fluorescence quenching Molecular docking Antioxidation Cytotoxicity Biomedicine Biological and Medical Physics, Biophysics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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