Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Russo, Andrew T. Brand, Ludwig |
| Copyright Year | 1999 |
| Abstract | Myelin basic protein (MBP) is a major component of myelin and plays a central role in the maintenance of its compact multilayered structure. Tryptophan fluorescence is sensitive to environmental factors such as polarity and rotational mobility and often reflects conformational changes in proteins. This work describes a detailed examination of the time-resolved emission properties of the single tryptophan of recombinant human myelin basic protein. Fluorescence decay curves were collected at two separate excitation wavelengths and at different wavelengths across the emission spectrum. The fitting parameters obtained with the aid of global analysis were combined with steady-state emission spectra collected from the same samples and are presented as decay-associated spectra (DAS) and time-resolved emission spectra (TRES). The effects of temperature and binding to anionic membranes on the decay of emission of MBP were investigated. The changes in fitted parameters and the appearance of DAS and TRES as a function of experimental conditions are interpreted in terms of variation in the local environment of the single tryptophan in MBP. The implications of the changes in local environment resulting from experimental treatments are discussed in the context of the overall conformation of MBP and are compared to structural and photophysical properties of MBP obtained from the central nervous system tissue of several species [P. Cavatorta, S. Giovanelli, A. Bobba, P. Riccio, and E. Quagliariello, Acta Neurol. (Napoli) 13, 162–169, (1991; P. Cavatorta, S. Giovanelli, A. Bobba, P. Riccio, A. G. Szabo, and E. Quagliarello, Biophys. J. 66, 1174–1179, 1994; P. Cavatorta, L. Masotti, A. G. Szabo, D. Juretic, P. Riccio, and E. Quagliariello, Cell Biophys. 13, 201–215, 1988]. |
| Starting Page | 333 |
| Ending Page | 342 |
| Page Count | 10 |
| File Format | |
| ISSN | 10530509 |
| Journal | Journal of Fluorescence |
| Volume Number | 9 |
| Issue Number | 4 |
| e-ISSN | 15734994 |
| Language | English |
| Publisher | Kluwer Academic Publishers-Plenum Publishers |
| Publisher Date | 1999-01-01 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Analytical Chemistry Biochemistry Biophysics/Biomedical Physics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|