NDLI: Preparation of K$^{+}$-Doped Core-Shell NaYF$_{4}$:Yb, Er Upconversion Nanoparticles and its Application for Fluorescence Immunochromatographic Assay of Human Procalcitonin

Content Provider	Springer Nature Link
Author	Tang, Jie Lei, Lijiang Feng, Hui Zhang, Hongman Han, Yuwang
Copyright Year	2016
Abstract	In the present study, we reported a convenient route to prepare well dispersed and functionalized K$^{+}$-doped core-shell upconversion nanoparticles (UCP) by layer-by-layer (LbL) assembly of polyelectrolytes. UCP was firstly transferred to aqueous phase using cationic surfactant cetyl trimethyl ammonium bromide (CTAB) via hydrophobic interaction without removing the existing oleic acid (OA). Then the positively charged hydrophilic UCP@CTAB was further alternately deposited with negatively charged [poly (sodium 4-styrenesulfonate)] (PSS), positively charged [poly (allylamine hydrochloride)] (PAH) and negatively charged [poly (acrylic acid)] (PAA). The final carboxyl functionalized UCP@CTAB@PSS@PAH@PAA was then conjugated with monoclonal antibody1 (AB1) of procalcitonin (PCT), resulting in successful detection of PCT antigens based on the immunochromatographic assay (ICA). Linear response was achieved from 0 to 10 ng/mL, and the lowest limit of detection (LLD) was 0.18 ng/mL.
Starting Page	2237
Ending Page	2246
Page Count	10
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	26
Issue Number	6
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2016-09-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Upconversion K$^{+}$-doped Core-shell Procalcitonin Layer-by-layer assembly Biomedicine Biological and Medical Physics, Biophysics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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