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| Content Provider | Springer Nature Link |
|---|---|
| Author | Tristão, Vivian Regina Carvalho, Fernando Felippe Gomes, Cinthia Zanini Miranda, Adriana Regina Vequi Suplicy, Cíntia C. Lamy, Maria Teresa Schor, Nestor Bellini, Maria Helena |
| Copyright Year | 2010 |
| Abstract | The progression to end-stage renal failure is independent of the initial pathogenic mechanism. Metabolic acidosis is a common consequence of chronic renal failure that results from inadequate ammonium excretion and decreased tubular bicarbonate reabsorption. Protoporphyrin IX (PpIX) is the immediate metabolic precursor of the heme molecule. The purpose of this study was to evaluate the levels of erythrocytes protoporphyrin IX at an animal model during progressive renal disease. A total of 36 eight-week-old male Wistar rats were divided into six groups: Normal, 4 and 8 weeks after 5/6 nephrectomy (NX). Renal function was evaluated by creatinine clearance and plasma creatinine levels. The autofluorescence of erythrocytes porphyrin of healthy and NX rats was analyzed using fluorescence spectroscopy. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and NX rats autofluorescence shape occurred in the 600–700 nm spectral region. A correlation was observed between emission band intensity at 635 nm and progression of renal disease. |
| Starting Page | 665 |
| Ending Page | 669 |
| Page Count | 5 |
| File Format | |
| ISSN | 10530509 |
| Journal | Journal of Fluorescence |
| Volume Number | 20 |
| Issue Number | 3 |
| e-ISSN | 15734994 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2010-02-23 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chronic renal failure Protoporphyrin Fluorescence Analytical Chemistry Biochemistry Biotechnology Biophysics and Biological Physics Biomedicine general |
| Content Type | Text |
| Resource Type | Article |
| Subject | Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology |
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