NDLI: Nonlinear Behavior of the Autofluorescence Intensity on the Surface of Light-Scattering Biotissues and its Theoretical Proof

Content Provider	Springer Nature Link
Author	Rogatkin, Dmitry Guseva, Irina Lapaeva, Liudmila
Copyright Year	2015
Abstract	In the up-to-date medical laser fluorescence spectroscopy (LFS) in vivo, there is a problem of quantification of fluorophores concentrations in optically-turbid biotissues by measurements of the laser induced autofluorescence flux on the surface of these tissues. One of the main problems is: whether the flux depends linearly or non-linearly on the concentration of fluorophores in tissues? The purpose of this work was both experimental and theoretical study of the character of dependencies between measured fluorescence intensities and fluorophores concentrations in optically-turbid media. In the experimental part of our study, measurements of the superficial fluorescence on phantoms at various known concentrations of fluorophores in them were carried out. As a result, experimental dependencies of registered intensities of the laser-induced autofluorescence emission were plotted against fluorophore concentrations. In the theoretical part of the study, the analytical solution for the fluorescence emission by Kokhanovsky’s method based on the well-known two-flux Kubelka-Munk approach (KMA) was used. In addition, in our study the Kokhanovsky’s method was modified by its association with our improved KMA, allowing us to receive exact analytical solutions for boundary intensities collected by optical probes. As a result, a set of theoretical curves describing the influence of fluorophore concentrations on the registered autofluorescence intensities was obtained, as well. Both experimental and theoretical results show a good qualitative agreement with each other. Also, these results demonstrate that the dependence of the fluorescence intensity on tissues’ optical properties and on the concentration of fluorophores in light-scattering tissues can be both nonlinear and non-monotonic.
Starting Page	917
Ending Page	924
Page Count	8
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	25
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2015-04-23
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Autofluorescence Flux Fluorophore Concentration Noninvasive Diagnostics Theoretical simulations Biomedicine general Biophysics and Biological Physics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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