NDLI: Evaluation of Sensitivity of Fluorescence-Based Asbestos Detection by Correlative Microscopy

Content Provider	Springer Nature Link
Author	Ishida, Takeri Alexandrov, Maxym Nishimura, Tomoki Minakawa, Kenji Hirota, Ryuichi Sekiguchi, Kiyoshi Kohyama, rihiko Kuroda, Akio
Copyright Year	2011
Abstract	Fluorescence microscopy (FM) has recently been applied to the detection of airborne asbestos fibers that can cause asbestosis, mesothelioma and lung cancer. In our previous studies, we discovered that the E. coli protein DksA specifically binds to the most commonly used type of asbestos, chrysotile. We also demonstrated that fluorescent-labeled DksA enabled far more specific and sensitive detection of airborne asbestos fibers than conventional phase contrast microscopy (PCM). However, the actual diameter of the thinnest asbestos fibers visualized under the FM platform was unclear, as their dimensions were below the resolution of optical microscopy. Here, we used correlative microscopy (scanning electron microscopy [SEM] in combination with FM) to measure the actual diameters of asbestos fibers visualized under the FM platform with fluorescent-labeled DksA as a probe. Our analysis revealed that FM offers sufficient sensitivity to detect chrysotile fibrils as thin as 30–35 nm. We therefore conclude that as an analytical method, FM has the potential to detect all countable asbestos fibers in air samples, thus approaching the sensitivity of SEM. By visualizing thin asbestos fibers at approximately tenfold lower magnifications, FM enables markedly more rapid counting of fibers than SEM. Thus, fluorescence microscopy represents an advanced analytical tool for asbestos detection and monitoring.
Starting Page	357
Ending Page	363
Page Count	7
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	22
Issue Number	1
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2011-09-21
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Asbestos detection Fluorescence Asbestos-binding protein Chrysotile Correlative microscopy DksA Biotechnology Analytical Chemistry Biochemistry Biophysics and Biological Physics Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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