NDLI: Native Fluorescence and Time Resolved Fluorescence Spectroscopic Characterization of Normal and Malignant Oral Tissues Under UV Excitation

Content Provider	Springer Nature Link
Author	Udayakumar, Kanniyappan Yuvaraj, Maharan Awad, Fathi Jayanth, Vadivel Aruna, Prakasa Rao Koteeswaran, Dornadula David, Munusamy Balu Ganesan, Singaravelu
Copyright Year	2013
Abstract	The present work aims to investigates the native fluorescence and time resolved fluorescence spectroscopic characterization of oral tissues under UV excitation. The fluorescence emission spectra of oral tissues at 280 nm excitation were obtained. From the spectra, it was observed that the alteration in the biochemical and morphological changes present in tissues. Subsequently, the Full width at Half Maximum (FWHM) of every individual spectra of 20 normal and 40 malignant subjects were calculated. The student’s t-test analysis reveals that the data were statistically significant (p = 0.001). The fluorescence excitation spectra at 350 nm emission of malignant tissues confirms the alteration in protein fluorescence with respect to normal counterpart. To quantify the observed spectral differences, the two ratio variables R$_{1}$ = I$_{275}$/I$_{310}$ and R$_{2}$ = I$_{310}$/I$_{328}$ were introduced in the excitation spectra. Among them, the Linear Discriminant Analysis (LDA) of R$_{1}$ reveals better classification with 86.4 % specificity and 82.5 % sensitivity. The fluorescence decay kinetics of oral tissues was obtained at 350 nm emission and it was found that the decay kinetics was triple exponential. Then the ROC analysis of fractional amplitudes and component lifetime reveals that the average lifetime shows 77 % sensitivity and 70 % specificity with the cut off value 4.85 ns. Briefly, the average lifetime exhibits better statistical significance when compared to fractional amplitudes and component lifetimes.
Starting Page	613
Ending Page	623
Page Count	11
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	24
Issue Number	2
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2013-11-30
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oral cancer Fluorescence Excited state kinetics Intrinsic fluorophores Tryptophan Biomedicine general Biophysics and Biological Physics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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