NDLI: Biophysical Properties of Phenyl Succinic Acid Derivatised Hyaluronic Acid

Content Provider	Springer Nature Link
Author	Neves Petersen, Maria Teresa Klitgaard, Søren Skovsen, Esben Petersen, Steffen B. Tømmeraas, Kristoffer Schwach Abdellaoui, Khadija
Copyright Year	2009
Abstract	Modification of hyaluronic acid (HA) with aryl succinic anhydrides results in new biomedical properties of HA as compared to non-modified HA, such as more efficient skin penetration, stronger binding to the skin, and the ability to blend with hydrophobic materials. In the present study, hyaluronic acid has been derivatised with the anhydride form of phenyl succinic acid (PheSA). The fluorescence of PheSA was efficiently quenched by the HA matrix. HA also acted as a singlet oxygen scavenger. Fluorescence lifetime(s) of PheSA in solution and when attached to the HA matrix has been monitored with ps resolved streak camera technology. Structural and fluorescence properties changes induced on HA-PheSA due to the presence of singlet oxygen were monitored using static light scattering (SLS), steady state fluorescence and ps time resolved fluorescence studies. SLS studies provided insight into the depolymerisation kinetics of PheSA derivatised HA matrix in the presence of singlet oxygen. Time resolved fluorescence studies grave insight into the dynamics of the reaction mechanisms induced on HA-PheSA by singlet oxygen. These studies provided insight into the medical relevance of PheSA derivatised HA: its capacity of scavenging singlet oxygen and of quenching PheSA fluorescence. These studies revealed that HA-PheSA is a strong quencher of electronic excited state PheSA and acts as a scavenger of singlet oxygen, thus medical applications of this derivatised form of HA may protect tissues and organs, such as skin, against reactive oxygen species damage.
Starting Page	483
Ending Page	492
Page Count	10
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	20
Issue Number	2
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2009-11-28
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fluorescence lifetime Hyaluronic acid Hyaluronan Fluorescence quenching Singlet oxygen scavenger Analytical Chemistry Biochemistry Biotechnology Biophysics and Biological Physics Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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