NDLI: Whole-Object Fluorescence Lifetime Setup for Efficient Non-Imaging Quantitative Intracellular Fluorophore Measurements

Content Provider	Springer Nature Link
Author	Turgeman, Lior Deutsch, Mordechai Fixler, Dror
Copyright Year	2012
Abstract	In the present study we introduce a Whole-Object Fluorescence Life Time (wo-FLT) measurement approach for ease and a relatively inexpensive method of tracing alterations in intracellular fluorophore distribution and in the physical-chemical features of the microenvironments hosting the fluorophore. Two common fluorophores, Rhodamine 123 and Acridine Orange, were used to stain U937 cells which were incubated, with and without either Carbonyl cyanide 3-chlorphenylhydrazon or the apoptosis inducer H$_{2}$O$_{2}$. The wo-FLT, which is a non-imaging quantitative measurement, was able to detect several fluorescence decay components and corresponding weights in a single cell resolution. Following cell treatment, both decay time and weight were altered. Results suggest that the prominent factor responsible for these alterations and in some cases to a shift in emission spectrum as well, is the intracellular fluorophore local concentration. In this study it was demonstrated that the proposed wo-FLT method is superior to color fluorescence based imaging in cases where the emission spectrum of a fluorophore remains unchanged during the investigated process. The proposed wo-FLT approach may be of particular importance when direct imaging is impossible.
Starting Page	875
Ending Page	882
Page Count	8
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	22
Issue Number	3
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2012-01-20
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fluorescence life time Fluorescence dye concentration Membrane potential Whole-object fluorescence life time measurements Analytical Chemistry Biophysics and Biological Physics Biomedicine general Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

5,9-Diaminodibenzo[a,j]phenoxazinium Chloride: A Rediscovered Efficient Long Wavelength Fluorescent Dye

Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging

Improved Routine Bio-Medical and Bio-Analytical Online Fluorescence Measurements Using Fluorescence Lifetime Resolution

Determination of Copper(II) Ion Concentration by Lifetime Measurements of Green Fluorescent Protein

Segmented Frequency-domain Fluorescence Lifetime Measurements: Minimizing the Effects of Photobleaching Within a Multi-component System

Quantitative Analysis of Intracellular Calcium and Mitochondrial Kinetic Fluorescence Changes in GSNO-induced Thymocyte Early Apoptosis

Fluorescence Lifetime Correlation Spectroscopy

An Analog Filter Approach to Frequency Domain Fluorescence Spectroscopy

Rotational Diffusion and Solvatochromic Correlation of Coumarin 6 Laser Dye

Whole-Object Fluorescence Lifetime Setup for Efficient Non-Imaging Quantitative Intracellular Fluorophore Measurements

Similar Documents

5,9-Diaminodibenzo[a,j]phenoxazinium Chloride: A Rediscovered Efficient Long Wavelength Fluorescent Dye

Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging

Improved Routine Bio-Medical and Bio-Analytical Online Fluorescence Measurements Using Fluorescence Lifetime Resolution

Determination of Copper(II) Ion Concentration by Lifetime Measurements of Green Fluorescent Protein

Segmented Frequency-domain Fluorescence Lifetime Measurements: Minimizing the Effects of Photobleaching Within a Multi-component System

Quantitative Analysis of Intracellular Calcium and Mitochondrial Kinetic Fluorescence Changes in GSNO-induced Thymocyte Early Apoptosis

Fluorescence Lifetime Correlation Spectroscopy

An Analog Filter Approach to Frequency Domain Fluorescence Spectroscopy

Rotational Diffusion and Solvatochromic Correlation of Coumarin 6 Laser Dye

Whole-Object Fluorescence Lifetime Setup for Efficient Non-Imaging Quantitative Intracellular Fluorophore Measurements