NDLI: Bioimaging of Peroxynitrite in MCF-7 Cells by a New Fluorescent Probe Rhodamine B Phenyl Hydrazide

Content Provider	Springer Nature Link
Author	Ambikapathi, Gopi Kempahanumakkagari, Suresh Kumar Ramappa Lamani, Babu Kuramkote Shivanna, Devaraju Bodagur Maregowda, Harish Gupta, Anushree Malingappa, Pandurangappa
Copyright Year	2013
Abstract	Peroxynitrite is a potent oxidizing and nitrating agent which has detrimental effects on cells by altering the structure and function of biomolecules present within. A fluorescent probe rhodamine B phenyl hydrazide (RBPH) has been proposed for peroxynitrite (ONOO$^{−}$) imaging in MCF-7 cells based on its oxidation property, which converts RBPH to pink colored and highly fluorescent rhodamine B. The fluorescence emission intensity of the rhodamine B produced in the above process is linearly related to the concentration of peroxynitrite. The method obeys Beer’s law in the concentration range 2–20 nM and the detection limit has been found to be 1.4 nM. The possible reaction mechanism of peroxynitrite with RBPH to produce rhodamine B has been discussed with spectroscopic evidence. The Probe is selective to the peroxynitrite in the pH range 6–8 which is near physiological pH. Fluorescence microscopic studies suggest that the probe is cell permeable and hence peroxynitrite was imaged in MCF-7 cells.
Starting Page	705
Ending Page	712
Page Count	8
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	23
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2013-03-12
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Peroxynitrite Rhodamine B phenyl hydrazide Fluorimetry Bioimaging MCF-7 cells Biomedicine general Biophysics and Biological Physics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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