NDLI: Role of the Carbohydrate Moiety and of α-L-Fucose in the Stabilization and the Dynamics of the Lens culinaris Agglutinin–Glycoprotein Complex. A Fluorescence Study

Content Provider	Springer Nature Link
Author	Albani, J. R. Debray, H. Vincent, M. Gallay, J.
Copyright Year	1997
Abstract	Interaction between the fluorescent Lens culinaris agglutinin–fluorescein complex (LCA-FITC) and two glycoproteins, lactotransferrin (LTF) and serotransferrin (STF), was studied. The two glycoproteins have the same glycan structures, with one difference: the lactotransferrin glycans contain a fucose residue α-1,6-linked to the N-acetylglucosamine residue involved in the N-glycosylamine linkage. Fluorescence intensity quenching of the LCA-FITC complex shows that affinity between LCA and lactotransferrin is 50 times higher than that between LCA and serotransferrin, the fucose playing a major role in this high affinity (K $_{a}$ is equal to 9.66 and 0.188 μM $^{−1}$ for the LCA–LTF complex and LCA–STF complex, respectively). Time-resolved anisotropy decay indicates that the rotational correlation time of LCA (20 ns) does not change to a large extent whether the glycoproteins are bound to LCA or not. This suggests that there is no extended physical contact between LCA and the glycoproteins. The interaction between LCA and the glycoproteins occurs likely only via the carbohydrate chains, the STF and the LTF rotating almost-freely in the vicinity of LCA, with the glycans as an anchor.
Starting Page	293
Ending Page	298
Page Count	6
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	7
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Analytical Chemistry Biochemistry Biophysics/Biomedical Physics
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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