NDLI: Regulatory interconnections of cyclooxygenase and inducible NO-Synthase in urinary bladder epithelial cells of the frog Rana temporaria under effect of bacterial stimuli

Please wait, while we are loading the content...

Regulatory interconnections of cyclooxygenase and inducible NO-Synthase in urinary bladder epithelial cells of the frog Rana temporaria under effect of bacterial stimuli

Effects of lipid physicochemical characteristics on interrelations between lipid composition and the mouse liver index

Functional state of adenylyl cyclase signaling system in rat testis and ovary under conditions of fasting

Oppositely directed actions of different doses of arginine-vasotocin and 1-deamino-arginine-vasotocin on sodium ion transport in skin of the frog Rana temporaria

Renal filtration and reabsorption of GFP in Rana temporaria: Effect of arginine-vasotocin

Odor as an element of subjective assessment of attractiveness of young males and females

Activity of cytochrome oxidase in centers of tectofugal and thalamofugal tracts of the visual system of pigeon Columbia livia

Membrane potential of mitochondria in hepatocytes of the river lamprey Lampetra fluviatilis at periods of metabolic depression and activity

The index of English names of research objects to Volume 46, 2010

Journal of Evolutionary Biochemistry and Physiology : Volume 46

Journal of Evolutionary Biochemistry and Physiology : Volume 45

Journal of Evolutionary Biochemistry and Physiology : Volume 44

Journal of Evolutionary Biochemistry and Physiology : Volume 43

Journal of Evolutionary Biochemistry and Physiology : Volume 42

Journal of Evolutionary Biochemistry and Physiology : Volume 41

Journal of Evolutionary Biochemistry and Physiology : Volume 40

Journal of Evolutionary Biochemistry and Physiology : Volume 39

Journal of Evolutionary Biochemistry and Physiology : Volume 38

Journal of Evolutionary Biochemistry and Physiology : Volume 37

Journal of Evolutionary Biochemistry and Physiology : Volume 36

Regulatory interconnections of cyclooxygenase and inducible NO-Synthase in urinary bladder epithelial cells of the frog Rana temporaria under effect of bacterial stimuli

Content Provider	Springer Nature Link
Author	Bakhteeva, V. T. Fedotov, T. M. Nikolaeva, S. D. Lavrova, E. A. Fok, E. M. Parva, R. G.
Copyright Year	2011
Abstract	Earlier we have shown that in epithelial cells of the frog urinary bladder under action of bacterial lipopolysaccharides (LPS) there is activated expression of inducible NO-synthase (iNOS) and there is increased the NO production, which can play an important role in providing protective cell reactions form pathogens. The goal of the present work consisted in study of cyclooxygenase (cOG) products and mechanisms of their regulatory effect on expression of iNOS under action of LPS. In experiments on urinary bladder epithelial cells on the frog Rana temporaria it has been shown that incubation of the cells for 21 h with LPS leads to a rise in production of PGE$_{2}$ and nitrites, stable NO metabolites. Inhibitor of iNOS 1400W decreased sharply production of nitrites, but did not affect the PGE$_{2}$ level. Both the basal and the LPS-stimulated level of PGE$_{2}$ and nitrites were inhibited in the presence of selective cOG inhibitors SC-560 (cOG-1) and NS-398 (cOG-2). The IC$_{50}$ value amounted to 90, 220 and 470 μM for NS-398, SC-560, and diclofenac (unspecific inhibitor of both isoforms), respectively. PGE$_{2}$ and butaprost, the EP$_{2}$-receptor agonist, but not agonists of EP$_{1}$/EP$_{3}$ or EP$_{1}$ receptors, partially eliminated the inhibitory action of diclofenac on production of nitrites. Action of PGE$_{2}$ was accompanied by an increase in the intracellular cAMP. Analysis of expression of iNOS mRNA in the epithelial cells incubated with LPS or LPS + inhibitor of cPG has shown the LPS-stimulated rise in expression of iNOS mRNA to decrease sharply in the presence of SC-560 or NS-398. Thus, the epithelial cells of the frog urinary bladder have the effectively functioning system of the congenital immune protection against bacterial pathogens, the most important component of this system being PGE$_{2}$ and NO. Analysis of mechanisms of regulatory interactions of cOG and iNOS indicates that in this cell type the main regulators of iNOS expression and of the nitrogen oxide level are products of the cOG catalytic activity.
Starting Page	28
Ending Page	36
Page Count	9
File Format	PDF
ISSN	00220930
Journal	Journal of Evolutionary Biochemistry and Physiology
Volume Number	47
Issue Number	1
e-ISSN	16083202
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2011-04-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cyclooxygenase inducible NO-synthase prostaglandin E$_{2}$ E. coli LPS regulation of expression Evolutionary Biology Animal Physiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Biochemistry Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Arginase activity in frog urinary bladder epithelial cells and its involvement in regulation of nitric oxide production

Study of NO-synthase activity and detection of NO-dependent antimicrobial effects in primary culture of frog urinary bladder epithelial cells

Regulation of urea permeability in frog urinary bladder by prostaglandin E2

Temporal peculiarities of responses of auditory system neurons of the frog Rana temporaria to tone bursts with different modulation frequencies

Nitric oxide inhibits arginine-vasotocin-induced increase of water osmotic permeability in frog urinary bladder

Regulation of Prostaglandin Biosynthesis by Nitric Oxide Is Revealed by Targeted Deletion of Inducible Nitric-oxide Synthase

Role of acetylcholine in Ca$^{2+}$-dependent regulation of functional activity of the frog Rana temporaria myocardium

Peculiarities of Ca$^{2+}$-regulation of functional activity of myocardium of frog Rana temporaria

Heterogeneity of Mechanisms of Olfactory Transduction in the Frog Rana temporaria

Regulatory interconnections of cyclooxygenase and inducible NO-Synthase in urinary bladder epithelial cells of the frog Rana temporaria under effect of bacterial stimuli

Similar Documents

Arginase activity in frog urinary bladder epithelial cells and its involvement in regulation of nitric oxide production

Study of NO-synthase activity and detection of NO-dependent antimicrobial effects in primary culture of frog urinary bladder epithelial cells

Regulation of urea permeability in frog urinary bladder by prostaglandin E2

Temporal peculiarities of responses of auditory system neurons of the frog Rana temporaria to tone bursts with different modulation frequencies

Nitric oxide inhibits arginine-vasotocin-induced increase of water osmotic permeability in frog urinary bladder

Regulation of Prostaglandin Biosynthesis by Nitric Oxide Is Revealed by Targeted Deletion of Inducible Nitric-oxide Synthase

Role of acetylcholine in Ca$^{2+}$-dependent regulation of functional activity of the frog Rana temporaria myocardium

Peculiarities of Ca$^{2+}$-regulation of functional activity of myocardium of frog Rana temporaria

Heterogeneity of Mechanisms of Olfactory Transduction in the Frog Rana temporaria

Regulatory interconnections of cyclooxygenase and inducible NO-Synthase in urinary bladder epithelial cells of the frog Rana temporaria under effect of bacterial stimuli